Inhibition of targeted platelet-derived growth factor receptor-α RNA interference into vitreous cavity for experimental proliferative vitreoretinopathy

Abstract

AIM:To observe the therapeutic effect of platelet-derived growth factor receptor-α RNA interference on inhibiting experimental proliferative vitreoretinopathy(PVR).METHODS:Different concentrations of PDGFR-α shRNA were blended with lip2000,and the final scale of PDGFR-α shRNA/lip2 000 complex was 1:1, 1:2 and 1:3 respectively(the concentration of PDGFR-α shRNA was respectively 2μg, 3μg and 4μg).All the complexes were cultivated in human retinal pigment epithelium(HRPE)for 24h after transfection and then respectively injected 0.1mL to rabbit vitreous cavity.Forty healthy adult rabbits were seleceted in this study and randomly divided into colored balanced salt solution(BSS)group(N), comprising lipofectamineTM2 000 HRPE cell dilution group(group A). The highest transduction efficiency of 1.0, 1.5 and 2.0μmol/L containing PDGFR-α receptors shRNA, lipofectamineTM2 000 of HRPE cell dilution were selected(respectively group B, C and D)with 8 eyes each, the right eyes as the experimental eye. The extent of PVR was observed by indirect ophthalmoscope; and slice staining situation was observed by immunohistochemistry.The fundus changes were observed by histopathology.RESULTS:The highest transduction efficiency of PDGFR-αshRNA/lip2 000 ratio was 1:2.The extent of PVR, the histopathology changes and the immunohistochemistry of PDGFR-α in group B,C and group D were significantly lower than that in group A, while group D was much lower than those of group B and C.CONCLUSION:PDGFR-αRNA interference could inhibit the formation of experimental PVR.

Keywords

• retinal pigment epithelium cell
• proliferative vitreoretinopathy
• platelet-derived growth factor receptor-α
• RNA interference