Effect of estrogens level on the fracture healing of tibia bone after ovariectomy and ovariohysterectomy in female dogs

Abstract

This study aimed to find out how estrogen affects fracture healing. Thirty adult bitches were employed, and they were put into three groups at random: First, second, and third. A mixture of ketamine and xylazine has been used to anesthetize experimental animals. Electrical drilling in the distal part of the tibial bone with a 10 mm diameter was done on the first group to create a hole in the tibia. The Tibia hole was created after ovariectomy in the second group but after ovariohysterectomy in the third group. Clinical, estrogen assay and histopathological characteristics were assessed in all groups. During the first week after surgery, clinical results showed that symptoms of inflammation in the surgical region were substantial in the first group, while the inflammation symptoms in the second group were similar to those in the first group; however, it was milder in the third group. In the second group, estrogen levels reduced modestly; however, in the third group, estrogen levels declined significantly in the fourth week after surgery. According to histopathological findings, the proliferation of osteoclasts and osteoblasts at 15 and 30 days after surgery is much more pronounced in the first and second groups than in the third groups. Osteocytes are also more common in the first group than in the second and third groups. According to the results of this study, fracture healing in the third group is much less quantitative and qualitative than in the other groups.

Keywords

• ovariectomy
• ovariohysterectomy
• estrogen
• fracture healing