Effect of ginger, chamomile, and green tea extracts on prostate cancer cells

Aysam M. Fayed; M.A. Abdelzaher; Neamah Hassoni Mahdi; Dina M.R. AlKhafaf; Mohamed AbdElRahman; Ahmed Khalid Aldhalmi; Zahraa Haleem Al-Qaim; Rania Abd Elmohsen Abo El nour; Heba G. Abdelzaher; Alaa Muqbil Alsirhani; Salwa El. Saied Morsi

Journal of Genetic Engineering and Biotechnology (Sep 2024)

Effect of ginger, chamomile, and green tea extracts on prostate cancer cells

Aysam M. Fayed,
M.A. Abdelzaher,
Neamah Hassoni Mahdi,
Dina M.R. AlKhafaf,
Mohamed AbdElRahman,
Ahmed Khalid Aldhalmi,
Zahraa Haleem Al-Qaim,
Rania Abd Elmohsen Abo El nour,
Heba G. Abdelzaher,
Alaa Muqbil Alsirhani,
Salwa El. Saied Morsi

Affiliations

Aysam M. Fayed: Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt; Medical Laboratory Techniques Department, College of Health and Medical Technique, Al-Mustaqbal University, Babylon 51001, Iraq
M.A. Abdelzaher: Environmental Science and Industrial Development Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, Egypt; Corresponding author.
Neamah Hassoni Mahdi: Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001 Babylon, Iraq
Dina M.R. AlKhafaf: Medical Laboratory Techniques Department, College of Health and Medical Technique, Al-Mustaqbal University, Babylon 51001, Iraq; College of Education, University of Al-Qadisiyah, Iraq
Mohamed AbdElRahman: College of Pharmacy, Al-Mustaqbal University, Babylon 51001, Iraq; Clinical Pharmacy Department, Badr University Hospital, Faculty of Medicine, Helwan University, Egypt
Ahmed Khalid Aldhalmi: College of Pharmacy, Al-Mustaqbal University, Babylon 51001, Iraq
Zahraa Haleem Al-Qaim: Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001 Babylon, Iraq
Rania Abd Elmohsen Abo El nour: Anesthesia Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001 Babylon, Iraq
Heba G. Abdelzaher: Department of Clinical Pharmacy, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt
Alaa Muqbil Alsirhani: Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka, Aljouf, Saudi Arabia
Salwa El. Saied Morsi: Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt

Journal volume & issue: Vol. 22, no. 3
p. 100395

Abstract

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Prostate cancer (PCa) is a prevalent form of malignancy in males and is a significant contributor to cancer-related mortality worldwide. Because of this, studying the molecular processes of PCa cell growth and death is crucial. Hence, it is imperative to conduct further research on the regulatory mechanism underlying the progression of PCa to enhance our comprehension and identify innovative therapeutic targets. The present study investigates an experimental approach that utilizes cost-effective and environmentally sustainable plant extracts sourced from Egypt, namely ginger, chamomile, and green tea, which have been solubilized in dimethyl sulfoxide (DMSO), then characterized by using different analytical means and techniques, such as HPLC and GC–MS. The present study employed MTT assay, ELISA, and qRT-PCR techniques to assess the possible impact of the investigated extracts on PCa in PC-3 cells. The findings indicate that ginger exhibited a noteworthy cytotoxic impact on PC-3. Remarkably, the treatment of PCa cells with ginger significantly increased relative lactate dehydrogenase (LDH) production compared to those treated with chamomile and green tea extracts. Autophagy may play a crucial role in the context of chemotherapy. Modifying autophagy through its induction or inhibition is a promising and innovative approach to control cancer progression. Accordingly, it was found that ginger extract affects protein expression levels of autophagy markers LC3B, ATg12, and pro‐apoptotic signaling, including the Caspase-3 signaling pathway. The ELISA findings revealed a significant rise in the average levels of IL-1β and IL-8 after a 12-hour interval. To conclude, it can be inferred that ginger extract possesses the capability to control the production of inflammatory cytokines. Alternatively, utilizing herbal remedies containing ginger as a viable and secure means of treating PCa as an anticancer agent is possible.

Published in Journal of Genetic Engineering and Biotechnology

ISSN: 1687-157X (Print); 2090-5920 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Biology (General): Genetics
Website: https://www.sciencedirect.com/journal/journal-of-genetic-engineering-and-biotechnology

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