Evolution of a General RNA-Cleaving FANA Enzyme

Yajun Wang; Arlene K. Ngor; Ali Nikoomanzar; John C. Chaput

doi:10.1038/s41467-018-07611-1

Nature Communications (Nov 2018)

Evolution of a General RNA-Cleaving FANA Enzyme

Yajun Wang,
Arlene K. Ngor,
Ali Nikoomanzar,
John C. Chaput

Affiliations

Yajun Wang: Department of Pharmaceutical Sciences, University of California
Arlene K. Ngor: Department of Pharmaceutical Sciences, University of California
Ali Nikoomanzar: Department of Pharmaceutical Sciences, University of California
John C. Chaput: Department of Pharmaceutical Sciences, University of California

DOI: https://doi.org/10.1038/s41467-018-07611-1
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

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Artificial genetic polymers (XNAs) have been explored for their nuclease activity, but XNAzymes have proven challenging to discover by in vitro selection. Here, the authors generate an efficient RNA-cleaving 2’-fluoroarabino nucleic acid enzyme (FANAzyme) by in vitro evolution using natural DNA polymerases.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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