Autoimmunity (Oct 2020)

Ficolin-1 and ficolin-3 polymorphisms and susceptibility to rheumatoid arthritis

  • Cristhine Pieczarka,
  • Fabiana Antunes Andrade,
  • Sandra Jeremias Catarino,
  • Kárita Cláudia Freitas Lidani,
  • Lorena Bavia,
  • Regina Tizzot,
  • Thelma Skare,
  • Iara Jose de Messias-Reason

DOI
https://doi.org/10.1080/08916934.2020.1809654
Journal volume & issue
Vol. 53, no. 7
pp. 400 – 407

Abstract

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Rheumatoid arthritis (RA) is an autoimmune disease, which compromises the synovial membrane resulting in chronic inflammation. Ficolins are key proteins of the lectin pathway of complement able to recognize pathogen-associated molecular patterns, apoptotic cells, and cellular debris mediating the clearance by phagocytes. High ficolin-1 and ficolin-3 levels have been observed in RA patients, however, the influence of polymorphisms in the FCN1 gene in RA is not completely established, while no study evaluated FCN3 gene polymorphisms in RA to date. We investigated the influence of FCN1 and FCN3 gene polymorphisms in the susceptibility and clinical presentation of RA. A total of 148 patients with RA and up to 160 controls from Southern Brazil were genotyped by sequence-specific PCR (PCR-SSP) for five FCN1 promoter polymorphisms (rs2989727, rs10120023, rs17039495, rs10117466, and rs10858293) and three FCN3 gene variants (rs532781899, rs28362807, and rs4494157). The FCN1 g.-542GG (rs10120023) genotype and g.-542G allele, were associated with increased susceptibility to RA (p = .025, OR = 1.69 [1.07–2.69]; p = .041, OR = 1.47 [1.02–2.12], respectively) and related to decreased FCN1 gene expression in whole blood (p A] polymorphism in the promoter region might contribute to RA susceptibility, probably by impacting FCN1 gene expression.

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