MicroRNA-562 negatively regulated c-MET/AKT pathway in the growth of glioblastoma cells

Abstract

Xiaohu Nie, Zhongzhou Su, Renfu Yan, Ai Yan, Sheng Qiu, Yue ZhouDepartment of Neurosurgery, Huzhou Central Hospital, Wuxing District, Huzhou, Zhejiang 313000, P.R. ChinaBackground: MicroRNA-562 (miR-562) has been found to possess anti-cancer function in certain tumors. However, the function of miR-562 in glioblastoma (GBM) is still not fully understood. Purpose: The aim at present study is to analyze the function of miR-562 and its possible target in GBM cells. Patients and methods: In the present study, a total of 80 GBM samples and 16 adjacent noncancerous tissues were used to examine the expression of miR-562 and c-MET. In order to gain a deep insight into the molecular network of miR-562 and c-MET in GBM, the miR-562 mimic and inhibitor were transfected into two GBM cell lines (U251 and U87), respectively. Meanwhile, lentiviral vector was used to mediate overexpression of c-MET. Cell proliferation was examined via Cell Counting Kit-8 (CCK-8) assays. Meanwhile, cell apoptosis was analyzed by Annexin V-FTTC/PI staining assay. Results: Our results indicated that the level of miR-562 was downregulated in GBM tissues and the expression of c-MET was upregulated in tumors. Cell proliferation analysis indicated that miR-562 was an anti-proliferation effector in GBM cells. Moreover, cell apoptosis analysis suggested the pro-apoptosis function of miR-562 in GBM cells. Conclusion: Our results demonstrated that miR-562 negatively regulated the c-MET/AKT signal pathway. In addition, caspase-3 might also serve as another target for miR-562 in GBM cells. This research not only obtained a deep understanding of miR-562 but also provided evidence in terms of developing new prognostic biomarker for GBM. Keywords: glioblastoma, miR-562, c-MET, AKT, p-AKT, caspase-3

Keywords

• Glioblastoma
• miR-562
• c-MET
• AKT
• p-AKT
• caspase-3