Characterization of sexual maturity-associated N6-methyladenosine in boar testes

Pengfei Zhang; Fei Zhang; Heming Sui; Xingyu Yang; Yiming Ji; Shenghao Zheng; Wei Li; Kun Cheng; Chonglong Wang; Jun Jiao; Xiaodong Zhang; Zubing Cao; Yunhai Zhang

doi:10.1186/s12864-024-10343-w

BMC Genomics (May 2024)

Characterization of sexual maturity-associated N6-methyladenosine in boar testes

Pengfei Zhang,
Fei Zhang,
Heming Sui,
Xingyu Yang,
Yiming Ji,
Shenghao Zheng,
Wei Li,
Kun Cheng,
Chonglong Wang,
Jun Jiao,
Xiaodong Zhang,
Zubing Cao,
Yunhai Zhang

Affiliations

Pengfei Zhang: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Fei Zhang: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Heming Sui: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Xingyu Yang: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Yiming Ji: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Shenghao Zheng: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Wei Li: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Kun Cheng: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Chonglong Wang: Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences
Jun Jiao: Anhui Haoyu Animal Husbandry Co., Ltd
Xiaodong Zhang: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Zubing Cao: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University
Yunhai Zhang: Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University

DOI: https://doi.org/10.1186/s12864-024-10343-w
Journal volume & issue: Vol. 25, no. 1
pp. 1 – 10

Abstract

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Abstract Background The health and size of the testes are crucial for boar fertility. Testicular development is tightly regulated by epigenetics. N6-methyladenosine (m6A) modification is a prevalent internal modification on mRNA and plays an important role in development. The mRNA m6A methylation in boar testicular development still needs to be investigated. Results Using the MeRIP-seq technique, we identify and profile m6A modification in boar testes between piglets and adults. The results showed 7783 distinct m6A peaks in piglets and 6590 distinct m6A peaks in adults, with 2,471 peaks shared between the two groups. Enrichment of GO and KEGG analysis reveal dynamic m6A methylation in various biological processes and signalling pathways. Meanwhile, we conjointly analyzed differentially methylated and expressed genes in boar testes before and after sexual maturity, and reproductive related genes (TLE4, TSSK3, TSSK6, C11ORF94, PATZ1, PHLPP1 and PAQR7) were identified. Functional enrichment analysis showed that differential genes are associated with important biological functions, including regulation of growth and development, regulation of metabolic processes and protein catabolic processes. Conclusion The results demonstrate that m6A methylation, differential expression and the related signalling pathways are crucial for boar testicular development. These results suggest a role for m6A modification in boar testicular development and provided a resource for future studies on m6A function in boar testicular development.

Published in BMC Genomics

ISSN: 1471-2164 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Biology (General): Genetics
Website: http://bmcgenomics.biomedcentral.com

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