Reversible S-nitrosylation of bZIP67 by peroxiredoxin IIE activity and nitro-fatty acids regulates the plant lipid profile
Inmaculada Sánchez-Vicente,
Pablo Albertos,
Carlos Sanz,
Brecht Wybouw,
Bert De Rybel,
Juan C. Begara-Morales,
Mounira Chaki,
Capilla Mata-Pérez,
Juan B. Barroso,
Oscar Lorenzo
Affiliations
Inmaculada Sánchez-Vicente
Department of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, Spain
Pablo Albertos
Department of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, Spain; Corresponding author
Carlos Sanz
Department Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa-CSIC, Campus Universidad Pablo de Olavide, Ctra Utrera km 1, 41013 Sevilla, Spain
Brecht Wybouw
Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium
Bert De Rybel
Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium
Juan C. Begara-Morales
Department of Experimental Biology, Facultad de Ciencias Experimentales, Campus Universitario “Las Lagunillas” s/n, Universidad de Jaén, E-23071 Jaén, Spain
Mounira Chaki
Department of Experimental Biology, Facultad de Ciencias Experimentales, Campus Universitario “Las Lagunillas” s/n, Universidad de Jaén, E-23071 Jaén, Spain
Capilla Mata-Pérez
Department of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, Spain
Juan B. Barroso
Department of Experimental Biology, Facultad de Ciencias Experimentales, Campus Universitario “Las Lagunillas” s/n, Universidad de Jaén, E-23071 Jaén, Spain
Oscar Lorenzo
Department of Botany and Plant Physiology, Instituto de Investigación en Agrobiotecnología (CIALE), Facultad de Biología, Universidad de Salamanca. C/ Río Duero 12, 37185 Salamanca, Spain; Corresponding author
Summary: Nitric oxide (NO) is a gasotransmitter required in a broad range of mechanisms controlling plant development and stress conditions. However, little is known about the specific role of this signaling molecule during lipid storage in the seeds. Here, we show that NO is accumulated in developing embryos and regulates the fatty acid profile through the stabilization of the basic/leucine zipper transcription factor bZIP67. NO and nitro-linolenic acid target and accumulate bZIP67 to induce the downstream expression of FAD3 desaturase, which is misregulated in a non-nitrosylable version of the protein. Moreover, the post-translational modification of bZIP67 is reversible by the trans-denitrosylation activity of peroxiredoxin IIE and defines a feedback mechanism for bZIP67 redox regulation. These findings provide a molecular framework to control the seed fatty acid profile caused by NO, and evidence of the in vivo functionality of nitro-fatty acids during plant developmental signaling.
