Tg6F ameliorates the increase in oxidized phospholipids in the jejunum of mice fed unsaturated LysoPC or WD[S]

Arnab Chattopadhyay; Mohamad Navab; Greg Hough; Victor Grijalva; Pallavi Mukherjee; Hannah R. Fogelman; Lin H. Hwang; Kym F. Faull; Aldons J. Lusis; Srinivasa T. Reddy; Alan M. Fogelman

Journal of Lipid Research (May 2016)

Tg6F ameliorates the increase in oxidized phospholipids in the jejunum of mice fed unsaturated LysoPC or WD[S]

Arnab Chattopadhyay,
Mohamad Navab,
Greg Hough,
Victor Grijalva,
Pallavi Mukherjee,
Hannah R. Fogelman,
Lin H. Hwang,
Kym F. Faull,
Aldons J. Lusis,
Srinivasa T. Reddy,
Alan M. Fogelman

Affiliations

Arnab Chattopadhyay: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Mohamad Navab: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Greg Hough: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Victor Grijalva: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Pallavi Mukherjee: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Hannah R. Fogelman: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Lin H. Hwang: Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Kym F. Faull: Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Aldons J. Lusis: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736; Human Genetics, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736; Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Srinivasa T. Reddy: To whom correspondence should be addressed; Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736; Molecular and Medical Pharmacology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736; Obstetrics and Gynecology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736
Alan M. Fogelman: Departments of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA 90095-1736

Journal volume & issue: Vol. 57, no. 5
pp. 832 – 847

Abstract

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Mouse chow supplemented with lysophosphatidylcholine with oleic acid at sn-1 and a hydroxyl group at sn-2 (LysoPC 18:1) increased LysoPC 18:1 in tissue of the jejunum of LDL receptor (LDLR)-null mice by 8.9 ± 1.7-fold compared with chow alone. Western diet (WD) contained dramatically less phosphatidylcholine 18:1 or LysoPC 18:1 compared with chow, but feeding WD increased LysoPC 18:1 in the jejunum by 7.5 ± 1.4-fold compared with chow. Feeding LysoPC 18:1 or feeding WD increased oxidized phospholipids in the jejunum by 5.2 ± 3.0-fold or 8.6 ± 2.2-fold, respectively, in LDLR-null mice (P < 0.0004), and 2.6 ± 1.5-fold or 2.4 ± 0.92-fold, respectively, in WT C57BL/6J mice (P < 0.0001). Adding 0.06‥ by weight of a concentrate of transgenic tomatoes expressing the 6F peptide (Tg6F) decreased LysoPC 18:1 in the jejunum of LDLR-null mice on both diets (P < 0.0001), and prevented the increase in oxidized phospholipids in the jejunum in LDLR-null and WT mice on both diets (P < 0.008). Tg6F decreased inflammatory cells in the villi of the jejunum, decreased dyslipidemia, and decreased systemic inflammation in LDLR-null and WT mice on both diets. We conclude that Tg6F reduces diet-induced inflammation by reducing the content of unsaturated LysoPC and oxidized phospholipids in the jejunum of mice.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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