Stem Cell Reports (Nov 2019)
A Comprehensive Resource for Induced Pluripotent Stem Cells from Patients with Primary Tauopathies
- Celeste M. Karch,
- Aimee W. Kao,
- Anna Karydas,
- Khadijah Onanuga,
- Rita Martinez,
- Andrea Argouarch,
- Chao Wang,
- Cindy Huang,
- Peter Dongmin Sohn,
- Kathryn R. Bowles,
- Salvatore Spina,
- M. Catarina Silva,
- Jacob A. Marsh,
- Simon Hsu,
- Derian A. Pugh,
- Nupur Ghoshal,
- Joanne Norton,
- Yadong Huang,
- Suzee E. Lee,
- William W. Seeley,
- Panagiotis Theofilas,
- Lea T. Grinberg,
- Fermin Moreno,
- Kathryn McIlroy,
- Bradley F. Boeve,
- Nigel J. Cairns,
- John F. Crary,
- Stephen J. Haggarty,
- Justin K. Ichida,
- Kenneth S. Kosik,
- Bruce L. Miller,
- Li Gan,
- Alison M. Goate,
- Sally Temple,
- Carolina Alquezar,
- Kathryn Bowles,
- David Butler,
- John F. Crary,
- Li Gan,
- Alison M. Goate,
- Stephen J. Haggarty,
- Israel Hernandez,
- Valerie Hennes,
- Cindy Huang,
- Justin K. Ichida,
- Martin Kampmann,
- Aimee W. Kao,
- Celeste M. Karch,
- Anna Karydas,
- Kenneth S. Kosik,
- Rita Martinez,
- Khadijah Onanuga,
- M. Catarina Silva,
- Sally Temple,
- Chao Wang
Affiliations
- Celeste M. Karch
- Department of Psychiatry, Washington University in St. Louis School of Medicine, 425 South Euclid Avenue, Campus Box 8134, St. Louis, MO 63110, USA; Corresponding author
- Aimee W. Kao
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Anna Karydas
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Khadijah Onanuga
- Neural Stem Cell Institute, 1 Discovery Drive, Rensselaer, NY 12144, USA
- Rita Martinez
- Department of Psychiatry, Washington University in St. Louis School of Medicine, 425 South Euclid Avenue, Campus Box 8134, St. Louis, MO 63110, USA
- Andrea Argouarch
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Chao Wang
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158, USA
- Cindy Huang
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158, USA
- Peter Dongmin Sohn
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158, USA
- Kathryn R. Bowles
- Ronald M. Loeb Center for Alzheimer's Disease, Departments of Neuroscience, Neurology and Genetics & Genomic Sciences, Icahn School of Medicine, New York, NY 10029, USA
- Salvatore Spina
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- M. Catarina Silva
- Chemical Neurobiology Laboratory, Center for Genomic Medicine, Departments of Neurology & Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
- Jacob A. Marsh
- Department of Psychiatry, Washington University in St. Louis School of Medicine, 425 South Euclid Avenue, Campus Box 8134, St. Louis, MO 63110, USA
- Simon Hsu
- Department of Psychiatry, Washington University in St. Louis School of Medicine, 425 South Euclid Avenue, Campus Box 8134, St. Louis, MO 63110, USA
- Derian A. Pugh
- Ronald M. Loeb Center for Alzheimer's Disease, Departments of Neuroscience, Neurology and Genetics & Genomic Sciences, Icahn School of Medicine, New York, NY 10029, USA
- Nupur Ghoshal
- Department of Neurology, Washington University in St. Louis, St. Louis, MO 63110, USA
- Joanne Norton
- Department of Psychiatry, Washington University in St. Louis School of Medicine, 425 South Euclid Avenue, Campus Box 8134, St. Louis, MO 63110, USA
- Yadong Huang
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158, USA
- Suzee E. Lee
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- William W. Seeley
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Panagiotis Theofilas
- Department of Pathology and Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
- Lea T. Grinberg
- Department of Pathology and Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
- Fermin Moreno
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Kathryn McIlroy
- Neural Stem Cell Institute, 1 Discovery Drive, Rensselaer, NY 12144, USA
- Bradley F. Boeve
- Department of Neurology, Mayo Clinic College of Medicine, Rochester, MN, USA
- Nigel J. Cairns
- Department of Neurology, Washington University in St. Louis, St. Louis, MO 63110, USA
- John F. Crary
- Ronald M. Loeb Center for Alzheimer's Disease, Departments of Neuroscience, Neurology and Genetics & Genomic Sciences, Icahn School of Medicine, New York, NY 10029, USA; Department of Pathology, Fishberg Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
- Stephen J. Haggarty
- Chemical Neurobiology Laboratory, Center for Genomic Medicine, Departments of Neurology & Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
- Justin K. Ichida
- Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
- Kenneth S. Kosik
- Department of Molecular Cellular and Developmental Biology, Neuroscience Research Institute, Biomolecular Science and Engineering Program, University of California, Santa Barbara, Santa Barbara, CA, USA
- Bruce L. Miller
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Li Gan
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158, USA
- Alison M. Goate
- Ronald M. Loeb Center for Alzheimer's Disease, Departments of Neuroscience, Neurology and Genetics & Genomic Sciences, Icahn School of Medicine, New York, NY 10029, USA
- Sally Temple
- Neural Stem Cell Institute, 1 Discovery Drive, Rensselaer, NY 12144, USA; Corresponding author
- Carolina Alquezar
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Kathryn Bowles
- Ronald M. Loeb Center for Alzheimer’s disease, Depts. of Neuroscience, Neurology and Genetics & Genomic Sciences, Icahn School of Medicine, New York, NY 10029, USA
- David Butler
- Neural Stem Cell Institute, 1 Discovery Drive, Rensselaer, NY 12144, USA
- John F. Crary
- Department of Pathology, Fishberg Department of Neuroscience, Friedman Brain Institute, Ronald M. Loeb Center for Alzheimer's Disease, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
- Li Gan
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158
- Alison M. Goate
- Ronald M. Loeb Center for Alzheimer’s disease, Depts. of Neuroscience, Neurology and Genetics & Genomic Sciences, Icahn School of Medicine, New York, NY 10029, USA
- Stephen J. Haggarty
- Chemical Neurobiology Laboratory, Center for Genomic Medicine, Departments of Neurology & Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
- Israel Hernandez
- Department of Molecular Cellular and Developmental Biology, Neuroscience Research Institute, Biomolecular Science and Engineering Program, University of California, Santa Barbara, Santa Barbara, CA, USA
- Valerie Hennes
- Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
- Cindy Huang
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158
- Justin K. Ichida
- Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
- Martin Kampmann
- Department of Biochemistry and Biophysics, Institute for Neurodegenerative Diseases and California Institute for Quantitative Biomedical Research, University of California, San Francisco, CA 94158, USA
- Aimee W. Kao
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Celeste M. Karch
- Department of Psychiatry, Washington University in St Louis, St Louis, MO 63110, USA
- Anna Karydas
- Division of Memory and Aging Center, Department of Neurology, University of California, San Francisco, San Francisco, CA 94158, USA
- Kenneth S. Kosik
- Department of Molecular Cellular and Developmental Biology, Neuroscience Research Institute, Biomolecular Science and Engineering Program, University of California, Santa Barbara, Santa Barbara, CA, USA
- Rita Martinez
- Department of Psychiatry, Washington University in St Louis, St Louis, MO 63110, USA
- Khadijah Onanuga
- Neural Stem Cell Institute, 1 Discovery Drive, Rensselaer, NY 12144, USA
- M. Catarina Silva
- Chemical Neurobiology Laboratory, Center for Genomic Medicine, Departments of Neurology & Psychiatry, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
- Sally Temple
- Neural Stem Cell Institute, 1 Discovery Drive, Rensselaer, NY 12144, USA
- Chao Wang
- Gladstone Institutes of Neurological Disease, Department of Neurology, Neuroscience Graduate Program, University of California, San Francisco, CA 94158
- Journal volume & issue
-
Vol. 13,
no. 5
pp. 939 – 955
Abstract
Summary: Primary tauopathies are characterized neuropathologically by inclusions containing abnormal forms of the microtubule-associated protein tau (MAPT) and clinically by diverse neuropsychiatric, cognitive, and motor impairments. Autosomal dominant mutations in the MAPT gene cause heterogeneous forms of frontotemporal lobar degeneration with tauopathy (FTLD-Tau). Common and rare variants in the MAPT gene increase the risk for sporadic FTLD-Tau, including progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD). We generated a collection of fibroblasts from 140 MAPT mutation/risk variant carriers, PSP, CBD, and cognitively normal controls; 31 induced pluripotent stem cell (iPSC) lines from MAPT mutation carriers, non-carrier family members, and autopsy-confirmed PSP patients; 33 genome engineered iPSCs that were corrected or mutagenized; and forebrain neural progenitor cells (NPCs). Here, we present a resource of fibroblasts, iPSCs, and NPCs with comprehensive clinical histories that can be accessed by the scientific community for disease modeling and development of novel therapeutics for tauopathies. : In this article, Karch, Temple and colleagues describe a resource of fibroblasts, patient-derived induced pluripotent stem cells, and genome engineered stem cells with comprehensive clinical histories that can be accessed by the scientific community for disease modeling and development of novel therapeutics for primary tauopathies. Keywords: tau, MAPT, fibroblasts, induced pluripotent stem cells, neural progenitor cells, tauopathy, frontotemporal dementia, progressive supranuclear palsy, corticobasal degeneration, CRISPR/Cas9
