Comparative Study of Low Level Laser, Acupuncture and Liquid Stitch Effects on Surgical Wound Healing in Rat Model

Abstract

The objective of this study was to evaluate the efficacy of low-level laser, acupuncture, and liquid stitch on surgical wound healing in a rat model. 40 male Wistar rats (200-300 gr) were used in this study. Under general anesthesia (ketamine/xylazine), surgical preparation on both sides of the spine was performed and a skin incision was made on each side (1.5 cm). 40 wounds were closed by 3-0 polypropylene suture material in a simple interrupted pattern and half with liquid stitch. 80 wounds were divided into eight groups of ten: in groups A and B wounds were closed with polypropylene suture material and liquid stitch with no other treatment. In groups C and D wounds closed with suture materials, and liquid stitch, respectively, received acupuncture. In groups E and F, wounds closed with suture materials, and liquid stitch, respectively, received a low-level laser. In groups G and H wounds were closed with suture materials and liquid stitch, respectively, and received both laser and acupuncture. Treatments were started the day after surgery and continued until day 10. All the groups were subdivided into two equal groups which were sacrificed on days five and ten after surgery. Skin samples were taken for histopathological evaluations (H & E, Masson’s trichrome). The results showed that the quality of wound healing in groups C, D, E, F, G, and H, were statistically superior to the groups A, and B. Groups G and H which received both treatments, had better results than groups C, D, E, and F, which received only one of the treatments. Low-level laser treatment showed better results in comparison with acupuncture treatment. There was no significant difference between groups A and B. It can be concluded that both acupuncture and low-level laser has pro-healing properties on surgical wounds.

Keywords

• wound healing
• low-level laser
• acupuncture
• liquid stitch
• rat