PGC1α Controls Sucrose Taste Sensitization in Drosophila
Qiao-Ping Wang,
Yong Qi Lin,
Mei-Ling Lai,
Zhiduan Su,
Lisa J. Oyston,
Teleri Clark,
Scarlet J. Park,
Thang M. Khuong,
Man-Tat Lau,
Victoria Shenton,
Yan-Chuan Shi,
David E. James,
William W. Ja,
Herbert Herzog,
Stephen J. Simpson,
G. Gregory Neely
Affiliations
Qiao-Ping Wang
School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Guangzhou 510275, China; The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia; Corresponding author
Yong Qi Lin
The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Mei-Ling Lai
School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Guangzhou 510275, China
Zhiduan Su
Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Lisa J. Oyston
The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Teleri Clark
The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Scarlet J. Park
Department of Neuroscience, The Scripps Research Institute, Jupiter, FL, USA
Thang M. Khuong
The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Man-Tat Lau
The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Victoria Shenton
The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
Yan-Chuan Shi
Neuroscience Division, Garvan Institute of Medical Research, St. Vincent’s Hospital, Darlinghurst, NSW 2010, Australia
David E. James
Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
William W. Ja
Department of Neuroscience, The Scripps Research Institute, Jupiter, FL, USA
Herbert Herzog
Neuroscience Division, Garvan Institute of Medical Research, St. Vincent’s Hospital, Darlinghurst, NSW 2010, Australia
Stephen J. Simpson
Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
G. Gregory Neely
The Dr. John and Anne Chong Laboratory for Functional Genomics, Charles Perkins Centre and School of Life & Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia; Molecular Evolution Laboratory, Centenary Institute, The University of Sydney, Sydney, NSW 2006, Australia; Corresponding author
Summary: Perceived palatability of food controls caloric intake. Sweet taste is the primary means of detecting the carbohydrate content of food. Surprisingly, sweet taste sensitivity is responsive to extrinsic factors like diet, and this occurs by unknown mechanisms. Here, we describe an unbiased proteomic investigation into sweet taste sensitivity in the fruit fly. We identify a dopamine/cyclic AMP (cAMP)/CREB axis acting within sweet taste neurons that controls taste perception but is largely dispensable for acute taste transduction. This pathway modulates sweet taste perception in response to both sensory- and nutrient-restricted diets and converges on PGC1α, a critical regulator of metabolic health and lifespan. By electrophysiology, we found that enhanced sucrose taste sensitivity was the result of heightened sweet taste intensity and that PGC1α was both necessary and sufficient for this effect. Together, we provide the first molecular insight into how diet-induced taste perception is regulated within the sweet taste neuron. : Wang et al. show that manipulation of diet composition can alter subsequent sweet taste sensitivity. This effect involves dopamine signaling pathways and PGC1α. These findings explain how diet composition can impact future sweet taste perception.
