Comprehensive sampling of the lung microbiome in early-stage non–small cell lung cancerCentral MessagePerspective

Rishindra M. Reddy, MD, MBA; Kiran Lagisetty, MD; Jules Lin, MD; Andrew C. Chang, MD; Abhinav Achreja, PhD; Nithya Ramnath, MD; Deepak Nagrath, PhD; Robert Dickson, MD; Frank Weinberg, MD, PhD

JTCVS Open (Feb 2024)

Comprehensive sampling of the lung microbiome in early-stage non–small cell lung cancerCentral MessagePerspective

Rishindra M. Reddy, MD, MBA,
Kiran Lagisetty, MD,
Jules Lin, MD,
Andrew C. Chang, MD,
Abhinav Achreja, PhD,
Nithya Ramnath, MD,
Deepak Nagrath, PhD,
Robert Dickson, MD,
Frank Weinberg, MD, PhD

Affiliations

Rishindra M. Reddy, MD, MBA: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Mich; Address for reprints: Rishindra M. Reddy, MD, MBA, Section of Thoracic Surgery, Department of Surgery, University of Michigan, TC2120/5344, 1500 East Medical Center Dr, Ann Arbor, MI 48109.
Kiran Lagisetty, MD: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Mich
Jules Lin, MD: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Mich
Andrew C. Chang, MD: Section of Thoracic Surgery, Department of Surgery, University of Michigan, Ann Arbor, Mich
Abhinav Achreja, PhD: Biomedical Engineering, University of Michigan, Ann Arbor, Mich
Nithya Ramnath, MD: Hematology/Oncology, Department of Internal Medicine, University of Michigan, Ann Arbor, Mich
Deepak Nagrath, PhD: Biomedical Engineering, University of Michigan, Ann Arbor, Mich
Robert Dickson, MD: Pulmonary/Critical Care, Department of Internal Medicine, University of Michigan, Ann Arbor, Mich
Frank Weinberg, MD, PhD: Hematology/Oncology, Department of Internal Medicine, University of Illinois-Chicago, Chicago, Ill

Journal volume & issue: Vol. 17
pp. 260 – 268

Abstract

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Objectives: Data are scarce on whether the composition of the lung microbiome (extending from the nasopharynx to the peripheral lung tissue) varies according to histology or grade of non–small cell lung cancer. We hypothesized that the composition of the lung microbiome would vary according to the histology and the grade of non–small cell lung cancer. Methods: We collected naso-oral and central lobar (cancer affected, ipsilateral unaffected, and contralateral unaffected) bronchoalveolar lavage fluid and brushing samples from patients with clinical early-stage lung cancer between July 2018 and February 2020 at a single academic center. We performed bacterial 16S rRNA sequencing and then compared clinical and pathologic findings with microbiome signatures. Results: Samples were collected from 28 patients. Microbial composition in affected lobes displayed unique enrichment of oropharyngeal bacterial species that was significantly different compared with that from the unaffected contralateral lobes; patients with chronic obstructive pulmonary disease had similar diversity to those without chronic obstructive pulmonary disease (P = .1312). The lung microbiome diversity in patients with adenocarcinoma was similar to those with squamous cell cancer (P = .27). There were no differences in diversity or composition in the unaffected lobes of patients with adenocarcinoma versus squamous cell cancer. There was a trend toward lower lung microbial diversity in poorly differentiated adenocarcinomas compared with well-differentiated adenocarcinomas (P = .08). Conclusions: The lung microbiota differs between cancer affected and unaffected lobes in the same patient. Furthermore, poorly differentiated lung cancers were associated with lower microbial diversity. Larger studies will be required to confirm these findings.

Published in JTCVS Open

ISSN: 2666-2736 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the circulatory (Cardiovascular) system; Medicine: Surgery
Website: https://www.journals.elsevier.com/jtcvs-open

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Abstract

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