Nature Communications (Jul 2021)

Binding of guide piRNA triggers methylation of the unstructured N-terminal region of Aub leading to assembly of the piRNA amplification complex

  • Xiawei Huang,
  • Hongmiao Hu,
  • Alexandre Webster,
  • Fan Zou,
  • Jiamu Du,
  • Dinshaw J. Patel,
  • Ravi Sachidanandam,
  • Katalin Fejes Toth,
  • Alexei A. Aravin,
  • Sisi Li

DOI
https://doi.org/10.1038/s41467-021-24351-x
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 17

Abstract

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PIWI protein contains arginine rich motifs that are post-translationally modified to symmetrically methylated arginine (sDMA) residues. Here the authors show that piRNA loading into Aub triggers sDMA modification which is recognized by Krimper to promote formation of Krimper-Aub-Ago3 complex for piRNA amplification in Drosophila.