Frontiers in Bioengineering and Biotechnology (Feb 2022)

Titanium Nanobowl-Based Nest-Like Nanofiber Structure Prepared at Room Temperature and Pressure Promotes Osseointegration of Beagle Implants

  • Lei Sun,
  • Lei Sun,
  • Xuzhuo Chen,
  • Haizhang Mu,
  • Yin Xu,
  • Ruiguo Chen,
  • Rong Xia,
  • Lunguo Xia,
  • Shanyong Zhang,
  • Shanyong Zhang

DOI
https://doi.org/10.3389/fbioe.2022.841591
Journal volume & issue
Vol. 10

Abstract

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Nest-like nanofiber structures have potential applications in surface modifications of titanium implants. In this study, nest-like nanofiber structures were prepared on a titanium surface at room temperature and pressure by using the nanobowl template-assisted method combined with alkali etching. The characterization and biocompatibility of this material were analyzed by cellular adhesion, death, CCK-8, ALP, and RT-PCR assays in vitro, and osseointegration was evaluated by micro-CT and fluorescent labeling in vivo. The results showed that this nest-like nanofiber structure has a firmer and asperate surface than nanotubes, which leads to better cellular adhesion, proliferation, and differentiation capacity. In a beagle alveolar bone implant model, the nest-like nanofiber structure showed a better osseointegration capacity. In conclusion, this nest-like nanofiber structure has potential applications in dental implantology.

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