Association between the 5-methyl tetrahydrofolate reductase (5-MTHFR) and integrin Β3 subunit (ITGB3) genes polymorphism and major depressive disorder

Abstract

Background and objectives: Major depression disorder (MDD) is a common condition and syndromic sickness with devastating psychological, social, and economic effects. In general, it is a widespread problem, and the prevalence of MDD varies by country. The genetic contribution to MDD is estimated to be around 35%. This study was done to explore the association between 5-methyl tetrahydrofolate reductase (5-MTHFR) and integrin β3 subunit (ITGB3) genes polymorphism for development of major depressive disorder. Materials and methods: The current study comprised thirty MDD patients, ranging in age from 18 to 52 years. All of the individuals were diagnosed using the Diagnostic and Statistical Manual of Mental Axis I Disorder in the outpatient departments of Taif Psychiatry Health Hospital (DSM-IV). The three polymorphic markers, C667T and A1298C of the 5-MTHFR gene and T1565C of the ITGB3 gene, were genotyped using the PCR-RFLP method. Results: The C667T single nucleotide polymorphism was found to be in heterozygous state in ten patients, while the A1298C single nucleotide polymorphism was not found in any of them. Twenty-one patients had the T1565C single nucleotide polymorphism in the ITGB3 gene, five of whom were homozygous state and 16 of whom were heterozygous state. Seven patients were discovered to have compound mutations in both genes. Interpretation & conclusion: The current findings could point to a link between MDD and the T allele of the C667T polymorphism in the 5-MTHFR gene and the C allele of the T1565C polymorphism in the ITGB3 gene.

Keywords

• Major depression disorder
• 5-MTHFR
• ITGB3
• Single nucleotide polymorphism
• PCR-RFLP