The genome of the southern short-horned tree dragon Acanthosaura meridiona Trivalairat, Sumontha, Kunya & Chaingkul, 2022 (Squamata, Draconinae) was analyzed using classical and molecular techniques to identify and study its chromosomal and repetitive elements
Program of Biology, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Thailand
Thaintip Kraiprom
Program of Biology, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Thailand
Somsak Buathip
Program of Biology, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Thailand
Sarun Jumrusthanasan
Biology Program, Department of Science, Faculty of Science,Buriram Rajabhat University, Muang, Buriram, Thailand
Sarawut Kaewsri
Biology Program, Faculty of Science, Buriram Rajabhat University, Buriram, Thailand
Nattasuda Donbundit
Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
Phichaya Buasriyot
Department of Health Sciences, Faculty of Science and Technology, Rajamangala University of Technology Suvarnabhumi, Nonthaburi, Mueang Nonthaburi 11000
Weera Thongnetr
Division of Biology, Department of Science, Faculty of Science and Technology, Rajamangala University of Technology Krungthep, Bangkok, Thailand
Sumalee Phimphan
Biology Program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun, Thailand
Alongklod Tanomtong
Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
The cytogenetics of the southern short-horned tree dragon (Acanthosaura meridiona) are not reported yet. This study describes the karyotype of Acanthosaura meridiona Trivalairat, Sumontha, Kunya & Chaingkul, 2022 from southern Thailand. We using Giemsa staining, Ag-NOR banding, and fluorescence in situ hybridization (FISH) techniques using microsatellites d(CA)15, d(TA)15, d(CGG)10, and d(CAA)10 probes to analyze the chromosome. The karyotype of the A. meridiona is 2n = 34 chromosomes (fundamental number of 46), of which 5 pairs were large metacentric chromosomes, 2 pairs small metacentric chromosomes, and 20 microchromosomes (chromosome formula: 2n=34=Lm10+ Sm4+20mi). There are no sex differences in karyotypes between males and females. The NORs loci were on pair 5 of the large metacentric macrochromosomes. The FISH technique showed d(CA)15 and d(CGG)10 repeats on specific regions microchromosomes, while signals of d(TA)15 and d(CAA)10 repeats interspersed on macro- and microchromosomes. This study is significant for enhances our comprehension of the evolutionary mechanism of agamid lizards and promotes the conservation of biodiversity in tropical rainforests.
