Frontiers in Cellular and Infection Microbiology (Jan 2024)

Urinary microbiota signatures associated with different types of urinary diversion: a comparative study

  • Yuchao Liu,
  • Yuchao Liu,
  • Yuchao Liu,
  • Jingcheng Zhang,
  • Jingcheng Zhang,
  • Haotian Chen,
  • Haotian Chen,
  • Wentao Zhang,
  • Wentao Zhang,
  • Ailiyaer Ainiwaer,
  • Ailiyaer Ainiwaer,
  • Shiyu Mao,
  • Shiyu Mao,
  • Xudong Yao,
  • Xudong Yao,
  • Xudong Yao,
  • Tianyuan Xu,
  • Tianyuan Xu,
  • Yang Yan,
  • Yang Yan,
  • Yang Yan

DOI
https://doi.org/10.3389/fcimb.2023.1302870
Journal volume & issue
Vol. 13

Abstract

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BackgroundRadical cystectomy and urinary diversion (UD) are gold standards for non-metastatic muscle-invasive bladder cancer. Orthotopic neobladder (or Studer), ileal conduit (or Bricker) and cutaneous ureterostomy (CU) are mainstream UD types. Little is known about urinary microbiological changes after UD. MethodsIn this study, urine samples were collected from healthy volunteers and patients with bladder cancer who had received aforementioned UD procedures. Microbiomes of samples were analyzed using 16S ribosomal RNA gene sequencing, and microbial diversities, distributions and functions were investigated and compared across groups. ResultsHighest urine microbial richness and diversity were observed in healthy controls, followed by Studer patients, especially those without hydronephrosis or residual urine, α-diversity indices of whom were remarkably higher than those of Bricker and CU groups. Studer UD type was the only independent factor favoring urine microbial diversity. The urine microflora structure of the Studer group was most similar to that of the healthy individuals while that of the CU group was least similar. Studer patients and healthy volunteers shared many similar urine microbial functions, while Bricker and CU groups exhibited opposite characteristics. ConclusionOur study first presented urinary microbial landscapes of UD patients and demonstrated the microbiological advantage of orthotopic neobladder. Microbiota might be a potential tool for optimization of UD management.

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