Acta Societatis Botanicorum Poloniae (Jan 2015)

Fractions of nucleic acids in seedlings and leaves of winter wheat in the vegetative and generative development phases

  • W. Filek

DOI
https://doi.org/10.5586/asbp.1973.015
Journal volume & issue
Vol. 42, no. 2
pp. 209 – 222

Abstract

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The composition of nucleic acid preparations isolated from seedlings and leaves of plants grown from vernalized and nonvernalized winter wheat grain was chromatographically investigated on a methylated albumin kieselguhr column (MAK column). The intensity of synthesis of the particular nucleic acid fractions was compared by the 32P-incorporation technique and the nucleotide composition of the newly synthesized nucleic acid fractions was determined. It was observed that the rate of 32P incorporation into the sRNA, rRNA+mRNA and TB-RNA fractions of the leaves of vernalized plants was about two times higher as compared with that in the nonvernalized control.