An alternative to MINFLUX that enables nanometer resolution in a confocal microscope

Abstract

Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale. Recently, the method called MINFLUX has achieved a ~10-fold improvement in spatial resolution over previously developed techniques, reaching molecular resolution with moderate photon counts. However, the technical complexity of this technique has hindered its widespread application. The new technique called RASTMIN provides equivalent resolution to MINFLUX while it can be implemented in standard scanning (confocal) microscopes.