The LC-MS/MS Identification and Analgesic and Wound Healing Activities of <i>Lavandula officinalis</i> Chaix: In Vivo and In Silico Approaches
Meryem Slighoua,
Mohamed Chebaibi,
Ismail Mahdi,
Fatima Ez-zahra Amrati,
Raffaele Conte,
Mary Anne W. Cordero,
Amal Alotaibi,
Hamza Saghrouchni,
Abdelkrim Agour,
Touria Zair,
Amina Bari,
Dalila Bousta
Affiliations
Meryem Slighoua
Laboratory of Biotechnology, Environment, Agro-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30050, Morocco
Mohamed Chebaibi
Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy of the Fez, Sidi Mohamed Ben Abdellah University, B.P. 1893, Km 22, Road of Sidi Harazem, Fez 30000, Morocco
Ismail Mahdi
AgroBioSciences Research Program, Mohammed VI Polytechnic University, Lot 660-Hay Moulay Rachid, Ben-Guerir 43150, Morocco
Fatima Ez-zahra Amrati
Laboratory of Biotechnology, Environment, Agro-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30050, Morocco
Raffaele Conte
Research Institute on Terrestrial Ecosystems (IRET)—CNR, Via Pietro Castellino 111, 80131 Naples, Italy
Mary Anne W. Cordero
Department of Basic Science, College of Medicine, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Amal Alotaibi
Department of Basic Science, College of Medicine, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Hamza Saghrouchni
Department of Biotechnology, Institute of Natural and Applied Sciences, Çukurova University, Balcali/Sariçam, Adana 01330, Turkey
Abdelkrim Agour
Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, P.O. Box 1796, Fez 30000, Morocco
Touria Zair
Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay 19 Ismail University, B.P. 11201, Meknes 50070, Morocco
Amina Bari
Laboratory of Biotechnology, Environment, Agro-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30050, Morocco
Dalila Bousta
Laboratory of Biotechnology, Environment, Agro-Food and Health (LBEAS), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30050, Morocco
We earlier emphasized in vivo the lavender plant’s (Lavandula officinalis Chaix.) anti-inflammatory and estrogenic activities and described the chemical compositions of its hydro-ethanolic (HE) extract. We used LC-MS/MS and GC-MS analyses to profile the phytochemical composition of the HE extract and to assess the analgesic and wound-healing effects of both the hydro-ethanolic (HE) and polyphenolic (LOP) extracts in vivo and in silico. The analgesic activity was studied using two methods: acetic acid and formalin injections in mice. The wound-healing activity was carried out over 25 days using a burn model in rats. In the in silico study, the polyphenols identified in the plant were docked in the active sites of three enzymes: casein kinase-1, cyclooxygenase-2, and glycogen synthase kinase-3β. The LC-MS/MS identified some phenolic compounds, mainly apigenin, catechin, and myricetin, and the GC-MS analysis revealed the presence of 19 volatile compounds with triazole, D-glucose, hydroxyphenyl, and D-Ribofuranose as the major compounds. The HE and LOP extracts showed significant decreases in abdominal writhes, and the higher licking time of the paw (57.67%) was observed using the LOP extract at 200 mg/kg. Moreover, both extracts showed high healing percentages, i.e., 99.31 and 92.88%, compared to the control groups, respectively. The molecular docking showed that myricetin, amentoflavone, apigenin, and catechin are the most active molecules against the three enzyme receptors. This study sheds light on the potential of L. officinalis Chaix as a source of natural products for pharmaceutical applications for analgesic purposes as well as their utility in promoting burn-healing activity.
