Extraction Optimization and Evaluation of the Antioxidant and α-Glucosidase Inhibitory Activity of Polysaccharides from <i>Chrysanthemum morifolium</i> cv. Hangju
Xiaoyan Hou,
Xia Huang,
Jianlong Li,
Guangyang Jiang,
Guanghui Shen,
Shanshan Li,
Qingying Luo,
Hejun Wu,
Meiliang Li,
Xingyan Liu,
Anjun Chen,
Meng Ye,
Zhiqing Zhang
Affiliations
Xiaoyan Hou
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Xia Huang
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Jianlong Li
College of Biomass Science and Engineering, Sichuan University, Chengdu 610000, China
Guangyang Jiang
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Guanghui Shen
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Shanshan Li
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Qingying Luo
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Hejun Wu
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Meiliang Li
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Xingyan Liu
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Anjun Chen
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Meng Ye
College of Forest, Sichuan Agricultural University, Chengdu 610000, China
Zhiqing Zhang
College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
In order to evaluate the antioxidant and α-glucosidase activities of polysaccharides from Chrysanthemum morifolium cv. Hangju (CMPs), the response surface methodology was applied to optimize the parameters for extraction progress of CMPs by ultrasound, with heat reflex extraction (HRE) performed as the control. The difference in the physicochemical properties of polysaccharides obtained by the two methods were also investigated. The maximum yields (8.29 ± 0.18%) of polysaccharides extracted by ultrasonic assisted extraction (UAE) were obtained under the optimized conditions of ultrasonic power 501 W, extraction time 19 min, and ratio of liquid-to-raw material 41 mL/g. Polysaccharides extracted by UAE possessed lower protein contents (2.56%) and higher uronic acids contents (7.08%) and low molecular weight fractions than that by HRE. No significant differences were found in monosaccharide composition and Fourier transform infrared (FT-IR) spectra of polysaccharides extracted by UAE and HRE, while polysaccharides by UAE possessed stronger antioxidant and α-glucosidase inhibitory activities. Therefore, UAE was an efficient way to obtain CMPs.
