Transcriptome-Wide Characterization of Seed Aging in Rice: Identification of Specific Long-Lived mRNAs for Seed Longevity

Bingqian Wang; Songyang Wang; Yuqin Tang; Lingli Jiang; Wei He; Qinlu Lin; Feng Yu; Long Wang; Long Wang

doi:10.3389/fpls.2022.857390

Frontiers in Plant Science (May 2022)

Transcriptome-Wide Characterization of Seed Aging in Rice: Identification of Specific Long-Lived mRNAs for Seed Longevity

Bingqian Wang,
Songyang Wang,
Yuqin Tang,
Lingli Jiang,
Wei He,
Qinlu Lin,
Feng Yu,
Long Wang,
Long Wang

Affiliations

Bingqian Wang: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, China
Songyang Wang: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, China
Yuqin Tang: National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Changsha, China
Lingli Jiang: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, China
Wei He: National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Changsha, China
Qinlu Lin: National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Changsha, China
Feng Yu: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, China
Long Wang: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, China
Long Wang: Longping Agricultural Science and Technology Huangpu Research Institute, Guangzhou, China

DOI: https://doi.org/10.3389/fpls.2022.857390
Journal volume & issue: Vol. 13

Abstract

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Various long-lived mRNAs are stored in seeds, some of which are required for the initial phase of germination and are critical to seed longevity. However, the seed-specific long-lived mRNAs involved in seed longevity remain poorly understood in rice. To identify these mRNAs in seeds, we first performed aging experiment with 14 rice varieties, and categorized them as higher longevity (HL) and lower longevity (LL) rice varieties in conventional rice and hybrid rice, respectively. Second, RNA-seq analysis showed that most genes showed similar tendency of expression changes during natural and artificial aging, suggesting that the effects of these two aging methods on transcription are comparable. In addition, some differentially expressed genes (DEGs) in the HL and LL varieties differed after natural aging. Furthermore, several specific long-lived mRNAs were identified through a comparative analysis of HL and LL varieties after natural aging, and similar sequence features were also identified in the promoter of some specific long-lived mRNAs. Overall, we identified several specific long-lived mRNAs in rice, including gibberellin receptor gene GID1, which may be associated with seed longevity.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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