Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27

Xiwei Ding; Kais Zakharia; Catherine D. Moser; Nellie A. Campbell; Chunling Hu; Nataliya Razumilava; Roongruedee Chaiteerakij; Hassan M. Shaleh; Patricia T. Greipp; Rondell P. Graham; Xiaoping Zou; Vishal S. Chandan; Lewis R. Roberts

doi:10.3390/cancers14205080

Cancers (Oct 2022)

Establishment and Characterization of a New Human Intrahepatic Cholangiocarcinoma Cell Line LIV27

Xiwei Ding,
Kais Zakharia,
Catherine D. Moser,
Nellie A. Campbell,
Chunling Hu,
Nataliya Razumilava,
Roongruedee Chaiteerakij,
Hassan M. Shaleh,
Patricia T. Greipp,
Rondell P. Graham,
Xiaoping Zou,
Vishal S. Chandan,
Lewis R. Roberts

Affiliations

Xiwei Ding: Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210000, China
Kais Zakharia: Division of Gastroenterology and Hepatology, University of Iowa, Iowa City, IA 52242, USA
Catherine D. Moser: Mayo Clinic and Mayo Clinic Cancer Center, Division of Gastroenterology and Hepatology, College of Medicine and Science, Rochester, MN 55905, USA
Nellie A. Campbell: Mayo Clinic and Mayo Clinic Cancer Center, Division of Gastroenterology and Hepatology, College of Medicine and Science, Rochester, MN 55905, USA
Chunling Hu: Mayo Clinic and Mayo Clinic Cancer Center, Division of Gastroenterology and Hepatology, College of Medicine and Science, Rochester, MN 55905, USA
Nataliya Razumilava: Mayo Clinic and Mayo Clinic Cancer Center, Division of Gastroenterology and Hepatology, College of Medicine and Science, Rochester, MN 55905, USA
Roongruedee Chaiteerakij: Mayo Clinic and Mayo Clinic Cancer Center, Division of Gastroenterology and Hepatology, College of Medicine and Science, Rochester, MN 55905, USA
Hassan M. Shaleh: Mayo Clinic and Mayo Clinic Cancer Center, Division of Gastroenterology and Hepatology, College of Medicine and Science, Rochester, MN 55905, USA
Patricia T. Greipp: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
Rondell P. Graham: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
Xiaoping Zou: Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210000, China
Vishal S. Chandan: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
Lewis R. Roberts: Mayo Clinic and Mayo Clinic Cancer Center, Division of Gastroenterology and Hepatology, College of Medicine and Science, Rochester, MN 55905, USA

DOI: https://doi.org/10.3390/cancers14205080
Journal volume & issue: Vol. 14, no. 20
p. 5080

Abstract

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Cholangiocarcinoma (CCA) is a highly lethal cancer arising from the biliary tract epithelium. The cancer biology of this neoplasm is not well understood. To date, only a few CCA cell lines have been reported, which were mostly developed from Asian patients. In this study, we report and characterize a new intrahepatic CCA cell line, LIV27, derived from a surgically resected tumor in a 67-year-old Caucasian woman with primary sclerosing cholangitis (PSC). LIV27 cells grow well in collagen-coated flasks or plates with a doubling time of 57.8 h at passage 14. LIV27 cells have high tumorigenicity in nude mice and stain positive for CK7 and CK19, markers that differentiate CCA from hepatocellular carcinoma. Karyotype analysis showed that LIV27 is aneuploid. We established a single-locus short tandem repeat profile for the LIV27 cell line. This newly established cell line will be a useful model for studying the molecular pathogenesis of, and developing novel therapies for, cholangiocarcinoma.

Published in Cancers

ISSN: 2072-6694 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.mdpi.com/journal/cancers/

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