Frontiers in Microbiology (Feb 2024)
Gut microbiota affects obesity susceptibility in mice through gut metabolites
- Yuhang Wen,
- Yuhang Wen,
- Yadan Luo,
- Yadan Luo,
- Hao Qiu,
- Hao Qiu,
- Baoting Chen,
- Baoting Chen,
- Jingrong Huang,
- Jingrong Huang,
- Shuya Lv,
- Shuya Lv,
- Yan Wang,
- Yan Wang,
- Jiabi Li,
- Jiabi Li,
- Lingling Tao,
- Lingling Tao,
- Bailin Yang,
- Bailin Yang,
- Ke Li,
- Ke Li,
- Lvqin He,
- Lvqin He,
- Manli He,
- Manli He,
- Qian Yang,
- Qian Yang,
- Zehui Yu,
- Zehui Yu,
- Wudian Xiao,
- Wudian Xiao,
- Mingde Zhao,
- Mingde Zhao,
- Xiaoxia Zou,
- Ruilin Lu,
- Congwei Gu,
- Congwei Gu
Affiliations
- Yuhang Wen
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Yuhang Wen
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Yadan Luo
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Yadan Luo
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Hao Qiu
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Hao Qiu
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Baoting Chen
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Baoting Chen
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Jingrong Huang
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Jingrong Huang
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Shuya Lv
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Shuya Lv
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Yan Wang
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Yan Wang
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Jiabi Li
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Jiabi Li
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Lingling Tao
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Lingling Tao
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Bailin Yang
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Bailin Yang
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Ke Li
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Ke Li
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Lvqin He
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Lvqin He
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Manli He
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Manli He
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Qian Yang
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Qian Yang
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Zehui Yu
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Zehui Yu
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Wudian Xiao
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Wudian Xiao
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Mingde Zhao
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Mingde Zhao
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Xiaoxia Zou
- Suining First People's Hospital, Suining, China
- Ruilin Lu
- Suining First People's Hospital, Suining, China
- Congwei Gu
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Congwei Gu
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2024.1343511
- Journal volume & issue
-
Vol. 15
Abstract
IntroductionIt is well-known that different populations and animals, even experimental animals with the same rearing conditions, differ in their susceptibility to obesity. The disparity in gut microbiota could potentially account for the variation in susceptibility to obesity. However, the precise impact of gut microbiota on gut metabolites and its subsequent influence on susceptibility to obesity remains uncertain.MethodsIn this study, we established obesity-prone (OP) and obesity-resistant (OR) mouse models by High Fat Diet (HFD). Fecal contents of cecum were examined using 16S rDNA sequencing and untargeted metabolomics. Correlation analysis and MIMOSA2 analysis were used to explore the association between gut microbiota and intestinal metabolites.ResultsAfter a HFD, gut microbiota and gut metabolic profiles were significantly different between OP and OR mice. Gut microbiota after a HFD may lead to changes in eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), a variety of branched fatty acid esters of hydroxy fatty acids (FAHFAs) and a variety of phospholipids to promote obesity. The bacteria g_Akkermansia (Greengene ID: 175696) may contribute to the difference in obesity susceptibility through the synthesis of glycerophosphoryl diester phosphodiesterase (glpQ) to promote choline production and the synthesis of valyl-tRNA synthetase (VARS) which promotes L-Valine degradation. In addition, gut microbiota may affect obesity and obesity susceptibility through histidine metabolism, linoleic acid metabolism and protein digestion and absorption pathways.
Keywords
- susceptibility to obesity
- gut microbiota
- untargeted metabolomics
- high-fat diet
- obesity-prone
- obesity-resistant
