LncRNA HIF1A-AS2 promotes triple-negative breast cancer progression and paclitaxel resistance via MRPS23 protein
Liangliang Min,
Lu Chen,
Da Huang,
Yulu Zhang,
Aihua You,
Xiaohua Yan,
Zhi-hua Li
Affiliations
Liangliang Min
Jiangxi Province Key Laboratory of Breast Diseases (No.2024SSY06221), Third Hospital of Nanchang, Jiangxi, 330009, China
Lu Chen
Jiangxi Province Key Laboratory of Breast Diseases (No.2024SSY06221), Third Hospital of Nanchang, Jiangxi, 330009, China
Da Huang
Jiangxi Province Key Laboratory of Breast Diseases (No.2024SSY06221), Third Hospital of Nanchang, Jiangxi, 330009, China
Yulu Zhang
Jiangxi Province Key Laboratory of Breast Diseases (No.2024SSY06221), Third Hospital of Nanchang, Jiangxi, 330009, China; Department of Breast Surgery, Third Hospital of Nanchang, Jiangxi, 330009, China
Aihua You
Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, 330004, China
Xiaohua Yan
Jiangxi Province Key Laboratory of Breast Diseases (No.2024SSY06221), Third Hospital of Nanchang, Jiangxi, 330009, China; School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330031, China
Zhi-hua Li
Jiangxi Province Key Laboratory of Breast Diseases (No.2024SSY06221), Third Hospital of Nanchang, Jiangxi, 330009, China; Department of Breast Surgery, Third Hospital of Nanchang, Jiangxi, 330009, China; Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, 330004, China; Corresponding author. Third Hospital of Nanchang, Jiangxi, 330009, China.
Dysregulation of lncRNAs is a critical factor in the migration and invasion of tumors. Here our study reveals that lncRNA HIF1A-AS2 is highly expressed in breast cancer tissues and various TNBC cell lines. Moreover, we present compelling evidence supporting the role of HIF1A-AS2 in promoting TNBC cell proliferation, metastasis, invasion, and resistance to paclitaxel treatment. Additionally, our transcriptome sequencing analysis identifies MRPS23 as a potential downstream target protein regulated by HIF1A-AS2 and knockdown of HIF1A-AS2 leads to decreased expression of MRPS23 in TNBC cells. Moreover, MRPS23 exhibits similar effects on enhancing cell proliferation, metastasis, invasion, and paclitaxel resistance in TNBC cells. Furthermore, downregulating HIF1A-AS2 suppresses the enhanced functionality observed in TNBC cells due to upregulated MRPS23 expression. These findings suggest that modulation of MRPS23 protein expression by HIF1A-AS2 may influence cellular processes and paclitaxel sensitivity in TNBC cells.
