The effect of cold water immersion after eccentric exercise on myogenic, inflammatory and muscle damage responses In FHL skeletal muscle in rats

Abstract

Purpose: cold water immersion is considered to accelerate the recovery from damaging exercise for athletes. Giventhe importance of satellite cells and the role of inflammatory responses in regeneration process, the purpose of thisstudy was to investigate the effect of cold water immersion on MyoD gene expression as an activation marker ofsatellite cells, CK as an indirect marker of damage and IL-6, IL-10 as inflammatory markers after eccentric exercisein male rats. Methods: 30 young and healthy male Wistar rats (Weight range= 300±10) were assigned randomly in5 groups each consisting of 6 subjects. It was an experimental research and subjects participated in eccentricexercise protocol (90min). Then they were compared in tow groups with and without cold water immersion(10min), in 3 and 48 hours after exercise in changes of MyoD gene expression in FHL skeletal muscle and the levelof serum CK,IL-6 and IL-10. We used Rest software for analyzing MyoD gene expression and independent T testfor analyzing serum data's. Results: Results showed that mRNA MyoD has been increased three hours aftereccentric exercise in cold water immersion group and the level of IL-6, IL-10 decreased significantly in that groupin compare with the group of three hours after eccentric exercise without cold water immersion. There is nosignificant change in CK. There was no significant changes in mRNA MyoD and serum CK,IL-6,IL-10 in 48 hourafter eccentric exercise and cold water immersion. Conclusion: Based on findings of the present study, cold waterduring recovery may cause the activation of satellite cells and decrease the inflammatory responses after eccentricexercise in early phase of damage.

Keywords

• damaging exercise
• satellite cells
• cold water immersion
• inflammation