Characterization and Hemocompatibility Assay of Microencapsulation Chitosan-Alginate Single Garlic Extract-loaded (MCA-SGE)

Abstract

Single garlic is a tropical plant with high bioactive compounds. Most bioactive compound found in single garlic is allicin but it has low bioavailability. Allicin was classified to reactive sulfur species (RSS) and has the ability to damage eukaryotic cells, for example is red blood cells (RBC). The damaged of RBC was minimized with a specific mechanism using the drug delivery system (DDS) with Microencapsulation Chitosan- Alginate (MCA). The aim of this study is to characterize and test the hemocompatibility of RBC. Single garlic extraction method using maceration with 70% ethanol. Optimal formulation of MCA-SGE determined using characterization with particle size analyzer (PSA) and hemocompatibility assay. PSA of MCA-SGE such as Z-Average (390.540± 11.460 nm), Polydispersity Index (PdI) (0.609 ± 0.011), and zeta potential (-23.067 ± 0.493 mV) shows that MCA-SGE categorized into optimal DDS. Hemocompatibility assay shows that MCA-SGE has low hemolysis percentage than SGE. The result of hemolysis percentage MCA-SGE does not cause the damage of RBC. Thus, it can be concluded that MCA-SGE was optimal increasing bioavailability of allicin compounds, hence MCASGE was compatible with RBC.