STAR Protocols (Mar 2022)

Normal and tumor-derived organoids as a drug screening platform for tumor-specific drug vulnerabilities

  • Camilla Calandrini,
  • Jarno Drost

Journal volume & issue
Vol. 3, no. 1
p. 101079

Abstract

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Summary: Patient-derived tumor organoids can be predictive of patient’s treatment responses, and normal tissue-derived organoids allow for drug toxicity testing. Combining both types of organoids therefore enables screening for tumor-specific drug vulnerabilities. Here, we provide a detailed protocol for organoid drug screening using, as proof-of-principle, patient-derived malignant rhabdoid tumor organoids. The protocol can be adapted for drug testing on any tumor and/or normal tissue-derived organoid culture.For complete details on the use and execution of this protocol, please refer to Calandrini et al. (2021).

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