Spermicidal Activity of the Safe Natural Antimicrobial Peptide Subtilosin

Infectious Diseases in Obstetrics and Gynecology. 2008;2008 DOI 10.1155/2008/540758

 

Journal Homepage

Journal Title: Infectious Diseases in Obstetrics and Gynecology

ISSN: 1064-7449 (Print); 1098-0997 (Online)

Publisher: Hindawi Publishing Corporation

LCC Subject Category: Medicine: Gynecology and obstetrics | Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy

Country of publisher: Egypt

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB, XML

 

AUTHORS

Michael L. Chikindas
Sebastian Faro
Alla A. Aroutcheva
Kenneth A. Feathergill
Sara E. Dover
Robert A. Anderson
Katia E. Sutyak

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 12 weeks

 

Abstract | Full Text

Bacterial vaginosis (BV), a condition affecting millions of women each year, is primarily caused by the gram-variable organism Gardnerella vaginalis. A number of organisms associated with BV cases have been reported to develop multidrug resistance, leading to the need for alternative therapies. Previously, we reported the antimicrobial peptide subtilosin has proven antimicrobial activity against G. vaginalis, but not against the tested healthy vaginal microbiota of lactobacilli. After conducting tissue sensitivity assays using an ectocervical tissue model, we determined that human cells remained viable after prolonged exposures to partially-purified subtilosin, indicating the compound is safe for human use. Subtilosin was shown to eliminate the motility and forward progression of human spermatozoa in a dose-dependent manner, and can therefore be considered a general spermicidal agent. These results suggest subtilosin would be a valuable component in topical personal care products aimed at contraception and BV prophylaxis and treatment.