Screening and Metabolomic Analysis of Lactic Acid Bacteria-Antagonizing <i>Pseudomonas aeruginosa</i>
Jianzhou Li,
Xiaohua Chen,
Ziyan Xie,
Lin Liang,
Anping Li,
Chao Zhao,
Yuxi Wen,
Zaixiang Lou
Affiliations
Jianzhou Li
College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Xiaohua Chen
Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences, Hengyang Normal University, Hengyang 421008, China
Ziyan Xie
Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences, Hengyang Normal University, Hengyang 421008, China
Lin Liang
Department of Life Sciences, Nanyue College of Hengyang Normal University, Hengyang 421008, China
Anping Li
College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Chao Zhao
College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Yuxi Wen
College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Zaixiang Lou
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Pseudomonas aeruginosa is a conditional Gram-negative pathogen that produces extracellular virulence factors that can lead to bloodstream invasion, severely harm tissues, and disseminate bacteria, ultimately leading to various diseases. In this study, lactic acid bacteria (LAB) with strong antagonistic ability against P. aeruginosa were screened, and the regulatory mechanism of LAB against P. aeruginosa was evaluated. The results showed that the three selected LAB strains had strong inhibition ability on the growth, biofilm formation, and pyocyanin expression of P. aeruginosa and a promoting effect on the expression of autoinducer-2. Among them, Lactipantibacillus plantarum (Lp. plantarum) LPyang is capable of affecting the metabolic processes of P. aeruginosa by influencing metabolic substances, such as LysoPC, oxidized glutathione, betaine, etc. These results indicate that LPyang reduces the infectivity of P. aeruginosa through inhibition of its growth, biofilm formation, pyocyanin expression, and regulation of its metabolome. This study provides new insights into the antagonistic activity of Lp. plantarum LPyang against P. aeruginosa.
