A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration

Feng Su; Christine Spee; Eduardo Araujo; Eric Barron; Mo Wang; Caleb Ghione; David  R. Hinton; Steven Nusinowitz; Ram Kannan; Srinivasa  T. Reddy; Robin Farias-Eisner

doi:10.3390/ijms20194807

International Journal of Molecular Sciences (Sep 2019)

A Novel HDL-Mimetic Peptide HM-10/10 Protects RPE and Photoreceptors in Murine Models of Retinal Degeneration

Feng Su,
Christine Spee,
Eduardo Araujo,
Eric Barron,
Mo Wang,
Caleb Ghione,
David R. Hinton,
Steven Nusinowitz,
Ram Kannan,
Srinivasa T. Reddy,
Robin Farias-Eisner

Affiliations

Feng Su: Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
Christine Spee: Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
Eduardo Araujo: Jules Stein Eye Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA
Eric Barron: The Stephen J. Ryan Initiative for Macular Research, Doheny Eye Institute, Los Angeles, CA 90033, USA
Mo Wang: The Stephen J. Ryan Initiative for Macular Research, Doheny Eye Institute, Los Angeles, CA 90033, USA
Caleb Ghione: Jules Stein Eye Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA
David R. Hinton: Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
Steven Nusinowitz: Jules Stein Eye Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA
Ram Kannan: Jules Stein Eye Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA
Srinivasa T. Reddy: Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
Robin Farias-Eisner: Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA

DOI: https://doi.org/10.3390/ijms20194807
Journal volume & issue: Vol. 20, no. 19
p. 4807

Abstract

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Age-related macular degeneration (AMD) is a leading cause of blindness in the developed world. The retinal pigment epithelium (RPE) is a critical site of pathology in AMD. Oxidative stress plays a key role in the development of AMD. We generated a chimeric high-density lipoprotein (HDL), mimetic peptide named HM-10/10, with anti-oxidant properties and investigated its potential for the treatment of retinal disease using cell culture and animal models of RPE and photoreceptor (PR) degeneration. Treatment with HM-10/10 peptide prevented human fetal RPE cell death caused by tert-Butyl hydroperoxide (tBH)-induced oxidative stress and sodium iodate (NaIO3), which causes RPE atrophy and is a model of geographic atrophy in mice. We also show that HM-10/10 peptide ameliorated photoreceptor cell death and significantly improved retinal function in a mouse model of N-methyl-N-nitrosourea (MNU)-induced PR degeneration. Our results demonstrate that HM-10/10 protects RPE and retina from oxidant injury and can serve as a potential therapeutic agent for the treatment of retinal degeneration.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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