Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)

Abstract

Summary: Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c+ cells and CD11c+-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in culture dishes, treating them with differentiation factors, and collecting cells after removal of undesirable populations. This protocol can be adapted for CD11c+ cells in different contexts, thus, serving as models for different diseases and to analyze cell-specific molecules.For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell biology
• Cell culture
• Cell isolation
• Flow cytometry/Mass cytometry
• Health sciences
• Immunology