STUDY CONCERNING PRODUCTION OF CELLULASE ENZYMES IN SOLID STATE CULTURES OF TRICHODERMA VIRIDE

Abstract

The hydrolysis of the lignocelluloses to fermentable sugars seems to be the main reason for the high producing cost of ethanol from lignocelluloses. The objective of this work is to test two strains of Trichoderma in liquid state and solid state cultures for cellulase production, and to compare the productivity and efficiency of the two systems of fermentation. Submerged liquid cultures (SLC) and solid state cultures (SSC) were carried out to compare the productivity of the two strains of Trichoderma. Comparing the productions of cellulases in the systems applied in this study, data indicate the system of solid state culture with flushing as the most efficient (660% more efficient in T. viride ATCC 13.631 SSC+f than in SLC and 455% more efficient in T. viride CMGB1 SSC+f than in SLC). Still, T. viride CMGB1 show a higher production (2160 FPU in SSC+f) than T. viride ATCC 13.631 (1880 FPU in SSC+fm) in laboratory conditions. These results recommend solid state cultures as systems for producing cellulases at lower price than liquid state cultures. These low cost cellulases can lower the price of ethanol produces from lignocellulosic biomass.

Keywords

• cellulase
• trichoderma
• ssf
• lsf
• cellulose hydrolysis