EFFECT OF FREQUENCY OF IMMERSION AND AIR INJECTION ON IN VITRO MULTIPLICATION OF Eucalyptus grandis x Eucalyptus urophylla IN TEMPORARY IMMERSION BIOREACTOR

Abstract

http://dx.doi.org/10.5902/1980509813321The objectives of this study were to evaluate different immersion frequencies (2, 4, 8 and 16 h) andexplants supports (filter paper and foam) of a Eucalyptus grandis x Eucalyptus urophylla clone insideRITA® bioreactors; and a ventilation system with additional air input coupled to the bioreactor containers.It was evaluated the explants fresh weight, number of shoots and hyperhydricity and in vitro axillary budmultiplication. The immersions every two and four hours and the filter paper showed higher growth andnumber of shoots, but caused a greater percentage of hyperhydric shoots. Under the conditions and systemtested, the additional air injection in the RITA® bioreactor did not influence the culture growth.

Keywords

• Propagação <i>in vitro</i>
• clonagem
• atmosfera gasosa
• hiper-hidricidade