Plant Secondary Metabolites on Efflux-Mediated Antibiotic Resistant <i>Stenotrophomonas Maltophilia</i>: Potential of Herbal-Derived Efflux Pump Inhibitors
Thi Huyen Thu Nguyen,
Ngoc Anh Thơ Nguyen,
Hai Dang Nguyen,
Thi Thu Hien Nguyen,
Mai Huong Le,
Minh Quan Pham,
Huu Nghi Do,
Kim Chi Hoang,
Serge Michalet,
Marie-Geneviève Dijoux-Franca,
Hoang Nam Pham
Affiliations
Thi Huyen Thu Nguyen
Doctoral School, University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 10072, Vietnam
Ngoc Anh Thơ Nguyen
UMR 5557, Ecologie Microbienne, CNRS, INRAe, VetagroSup, UCBL, Université de Lyon, 43 Boulevard du 11 Novembre, F-69622 Villeurbanne, France
Hai Dang Nguyen
Department of Academic Affairs, University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 10072, Vietnam
Thi Thu Hien Nguyen
Institute of Biological and Food Technology, Hanoi Open University, B101 Nguyen Hien, Hai Ba Trung, Hanoi 11615, Vietnam
Mai Huong Le
Institute of Natural Products Chemistry, 1H building, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 10072, Vietnam
Minh Quan Pham
Institute of Natural Products Chemistry, 1H building, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 10072, Vietnam
Huu Nghi Do
Institute of Natural Products Chemistry, 1H building, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 10072, Vietnam
Kim Chi Hoang
Institute of Natural Products Chemistry, 1H building, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 10072, Vietnam
Serge Michalet
UMR 5557, Ecologie Microbienne, CNRS, INRAe, VetagroSup, UCBL, Université de Lyon, 43 Boulevard du 11 Novembre, F-69622 Villeurbanne, France
Marie-Geneviève Dijoux-Franca
UMR 5557, Ecologie Microbienne, CNRS, INRAe, VetagroSup, UCBL, Université de Lyon, 43 Boulevard du 11 Novembre, F-69622 Villeurbanne, France
Hoang Nam Pham
Department of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Cau Giay, Hanoi 10072, Vietnam
During the process of adapting to metal contamination, plants produce secondary metabolites that have the potential to modulate multidrug-resistant (MDR) phenotypes; this is achieved by inhibiting the activity of efflux pumps to reduce the minimum inhibitory concentrations (MICs) of antimicrobial substrates. Our study evaluated the effect of secondary metabolites of belowground parts of Pteris vittata L. and Fallopia japonica, two metal-tolerant plants from northern Vietnam, on six antibiotic-resistant Stenotrophomonas maltophilia strains possessing efflux pump resistance mechanisms that were isolated from soil and clinical samples. The chemical composition of aqueous and dichloromethane (DCM) fractions extracted from P. vittata and F. japonica was determined using UHPLC-DAD-ESI/QTOF analysis. The antibacterial and efflux pump inhibitory activities of the four fractions were evaluated for the six strains (K279a, 0366, BurA1, BurE1, PierC1, and 502) using a microdilution assay at fraction concentrations of 62.5, 125, and 250 μg/mL. The DCM fraction of F. japonica exhibited remarkable antibacterial activity against strain 0366, with a MIC of 31.25 μg/mL. Furthermore, this fraction also significantly decreased gentamicin MIC: four-fold and eight-fold reductions for BurA1 and BurE1 strains, respectively (when tested at 250 μg/mL), and two-fold and eight-fold reductions for K279a and BurE1 strains, respectively (when tested at 125 μg/mL). Pure emodin, the main component identified in the DCM fraction of F. japonica, and sennidine A&B only reduced by half the MIC of gentamicin (when tested at 30 μg/mL). Our results suggest that the DCM fraction components of F. japonica underground parts may be potential candidates for new bacterial efflux pump inhibitors (EPIs).
