Determination of concentration of the pseudomycelles from fermentation broth via spectroscopy and Neubauer chamber

Abstract

The present work presents an alternative technique to obtain the concentration of pseudomycelles as number of pseudomycelles/sample volume, obtained by Neubauer chamber, as function of absorbance from spectroscopy. Samples of broth from fermentation via bacteria Streptomyces tsukubaensis were analyzed, making it possible to obtain a linear relationship between the number of pseudomycelles/ sample volume and absorbance, with determination coefficient of 0.995. In addition, a morphological analysis of the unfermented pseudomycelles was performed using scanning electron microscopy (SEM) at the magnifications of 20, 2000, 5000, 10000 and 20000 x. DOI: http://dx.doi.org/10.5281/zenodo.3987017

Keywords

• Neubauer chamber
• Pseudomycelles concentration
• Spectroscopy
• Absorbance
• Fermentation