Heterologous expression of heat-shock protein PpHSP70 improves high temperature and drought tolerance in rice
Shu-Yan Kou,
Zhi-Gang Wu,
Hong-Yu Li,
Xin Chen,
Wei-Hua Liu,
Ping-Rong Yuan,
Zhen-Hua Zhu,
Xu Yang,
Hua-Hui Li,
Ping Huang,
Li Liu
Affiliations
Shu-Yan Kou
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China
Zhi-Gang Wu
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China
Hong-Yu Li
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, China
Xin Chen
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, China
Wei-Hua Liu
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China
Ping-Rong Yuan
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China
Zhen-Hua Zhu
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China
Xu Yang
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China
Hua-Hui Li
Yunnan Agricultural Technology Extension Station, Kunming, Yunnan, China
Ping Huang
Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China; Corresponding authors.
Li Liu
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, China; Corresponding authors.
The research investigated the potential of heterologous expression of the heat-shock protein PpHSP70 in enhancing high temperature and drought tolerance in rice. After screening the whole genome sequence of Physcomitrium patens in the Phytzome database, 30 members of the HSP70 gene family were obtained. Their expression and the tissue expression patterns under abiotic stressors of high temperature, drought, and salt were analyzed. During the four-leaf stage of rice, overexpressed materials were subjected to a temperature of 45 °C for 20 h and to drought conditions for one week. After processing, 5 overexpression PpHSP70 materials survived against high temperature, and 7 materials survived against drought, while all wild-type materials KY131 died after the treatment. The results demonstrated that overexpression of PpHSP70 significantly improved both high-temperature tolerance and drought. This study provides valuable insights into the utilization of heterologous expression for enhancing crop tolerance to abiotic stressors.
