Nature Communications (Feb 2017)

CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor

  • Tyler Funnell,
  • Shinya Tasaki,
  • Arusha Oloumi,
  • Shinsuke Araki,
  • Esther Kong,
  • Damian Yap,
  • Yusuke Nakayama,
  • Christopher S. Hughes,
  • S.-W. Grace Cheng,
  • Hirokazu Tozaki,
  • Misa Iwatani,
  • Satoshi Sasaki,
  • Tomohiro Ohashi,
  • Tohru Miyazaki,
  • Nao Morishita,
  • Daisuke Morishita,
  • Mari Ogasawara-Shimizu,
  • Momoko Ohori,
  • Shoichi Nakao,
  • Masatoshi Karashima,
  • Masaya Sano,
  • Aiko Murai,
  • Toshiyuki Nomura,
  • Noriko Uchiyama,
  • Tomohiro Kawamoto,
  • Ryujiro Hara,
  • Osamu Nakanishi,
  • Karey Shumansky,
  • Jamie Rosner,
  • Adrian Wan,
  • Steven McKinney,
  • Gregg B. Morin,
  • Atsushi Nakanishi,
  • Sohrab Shah,
  • Hiroyoshi Toyoshiba,
  • Samuel Aparicio

DOI
https://doi.org/10.1038/s41467-016-0008-7
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 16

Abstract

Read online

The phosphorylation of serine/arginine-rich proteins by CDC-like kinase is a central regulatory mechanism for RNA splicing reactions. Here, the authors synthesize a novel small molecule CLK inhibitor and map CLK-responsive alternative splicing events and discover an effect on conjoined gene transcription.