Frontiers in Microbiology (Sep 2021)
A Cross-Reactive Monoclonal Antibody Against Neuraminidases of Both H9N2 and H3N2 Influenza Viruses Shows Protection in Mice Challenging Models
- Fei Wang,
- Fei Wang,
- Zhimin Wan,
- Zhimin Wan,
- Zhimin Wan,
- Jinsen Wu,
- Jinsen Wu,
- Yajuan Wang,
- Yajuan Wang,
- Hui Fu,
- Hui Fu,
- Hongxia Shao,
- Hongxia Shao,
- Hongxia Shao,
- Kun Qian,
- Kun Qian,
- Kun Qian,
- Wei Gao,
- Wei Gao,
- Wei Gao,
- Jianqiang Ye,
- Jianqiang Ye,
- Jianqiang Ye,
- Aijian Qin,
- Aijian Qin,
- Aijian Qin
Affiliations
- Fei Wang
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Fei Wang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Zhimin Wan
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Zhimin Wan
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Zhimin Wan
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China
- Jinsen Wu
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Jinsen Wu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Yajuan Wang
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Yajuan Wang
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Hui Fu
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Hui Fu
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Hongxia Shao
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Hongxia Shao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Hongxia Shao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China
- Kun Qian
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Kun Qian
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Kun Qian
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China
- Wei Gao
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Wei Gao
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Wei Gao
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Jianqiang Ye
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Jianqiang Ye
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China
- Aijian Qin
- Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, Yangzhou, China
- Aijian Qin
- Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China
- Aijian Qin
- Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2021.730449
- Journal volume & issue
-
Vol. 12
Abstract
Neuraminidases (NAs) of H9N2 avian influenza virus (AIV) and H3N2 human seasonal influenza virus (HSIV) share similar antigenic structures. However, there are few reports on epitopes shared by these two NAs. We previously reported a monoclonal antibody (mAb) 1G8 against the NA of H9N2 AIV with neuraminidase inhibition (NI) ability. In this study, 1G8 was shown to cross-react with and inhibit the NA of H3N2 HSIV. In a passive transfer experiment, 1G8 provided protection to mice challenged with rescued H1N2 viruses carrying H9N2 NA or H3N2 NA. Mutation at amino acid position 199 was also selected and proved to be crucial for H3N2 HSIV to escape from mAb 1G8. Moreover, we found that residue 199 contributed to inducing broad protective antibodies without the influence of the N-linked glycosylation at amino acid position 200 in NAs. Residues as residue 199, which are not shielded by glycosylation modification, would form ideal epitopes for developing universal vaccine and protective antibodies.
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