Nature Communications (Jun 2020)

Quantifying CDK inhibitor selectivity in live cells

  • Carrow I. Wells,
  • James D. Vasta,
  • Cesear R. Corona,
  • Jennifer Wilkinson,
  • Chad A. Zimprich,
  • Morgan R. Ingold,
  • Julie E. Pickett,
  • David H. Drewry,
  • Kathryn M. Pugh,
  • Marie K. Schwinn,
  • Byounghoon (Brian) Hwang,
  • Hicham Zegzouti,
  • Kilian V. M. Huber,
  • Mei Cong,
  • Poncho L. Meisenheimer,
  • Timothy M. Willson,
  • Matthew B. Robers

DOI
https://doi.org/10.1038/s41467-020-16559-0
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Cyclin-dependent kinase (CDK) inhibitors are widely used both in the clinic and for basic research aimed at dissecting the specific cellular functions of specific CDKs. Here, the authors report the development of a panel of fluorescent reporter probes and provide a comprehensive profile of the inhibitory activity of several CDK inhibitors towards all 21 CDKs in living cells.