IUCrJ (May 2019)
Long-wavelength native-SAD phasing: opportunities and challenges
- Shibom Basu,
- Vincent Olieric,
- Filip Leonarski,
- Naohiro Matsugaki,
- Yoshiaki Kawano,
- Tomizaki Takashi,
- Chia-Ying Huang,
- Yusuke Yamada,
- Laura Vera,
- Natacha Olieric,
- Jerome Basquin,
- Justyna A. Wojdyla,
- Oliver Bunk,
- Kay Diederichs,
- Masaki Yamamoto,
- Meitian Wang
Affiliations
- Shibom Basu
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Vincent Olieric
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Filip Leonarski
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Naohiro Matsugaki
- Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Tsukuba, 305-0801, Japan
- Yoshiaki Kawano
- Advanced Photon Technology Division, RIKEN SPring-8 Center, Hyogo 679-5148, Japan
- Tomizaki Takashi
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Chia-Ying Huang
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Yusuke Yamada
- Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Tsukuba, 305-0801, Japan
- Laura Vera
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Natacha Olieric
- Laboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen, PSI 5232, Switzerland
- Jerome Basquin
- Department of Biochemistry, Max Planck Institute of Biochemistry, Munich, Germany
- Justyna A. Wojdyla
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Oliver Bunk
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- Kay Diederichs
- Department of Biology, University of Konstanz, Konstanz, 78457, Germany
- Masaki Yamamoto
- Advanced Photon Technology Division, RIKEN SPring-8 Center, Hyogo 679-5148, Japan
- Meitian Wang
- Swiss Light Source, Paul Scherrer Institut, Villigen PSI, 5232, Switzerland
- DOI
- https://doi.org/10.1107/S2052252519002756
- Journal volume & issue
-
Vol. 6,
no. 3
pp. 373 – 386
Abstract
Native single-wavelength anomalous dispersion (SAD) is an attractive experimental phasing technique as it exploits weak anomalous signals from intrinsic light scatterers (Z 3 Å are discussed.
Keywords
- single-wavelength anomalous dispersion
- native-SAD phasing
- Se/S-SAD
- UV-laser cutting
- crystal shaping
- spherical crystals
- absorption correction
- anomalous scattering factor
- structure determination
