<i>Pholiota nameko</i> Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts
His Lin,
Kuan-Chen Cheng,
Jer-An Lin,
Liang-Po Hsieh,
Chun-Hsu Chou,
Yu-Ying Wang,
Ping-Shan Lai,
Po-Cheng Chu,
Chang-Wei Hsieh
Affiliations
His Lin
Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Road, South Dist., Taichung City 40227, Taiwan
Kuan-Chen Cheng
Institute of Biotechnology, National Taiwan University, Taipei 10617, Taiwan
Jer-An Lin
Graduate Institute of Food Safety, National Chung Hsing University, 145 Xingda Road, South Dist., Taichung City 40227, Taiwan
Liang-Po Hsieh
Department of Neurology, Cheng Ching General Hospital, Taichung 40764, Taiwan
Chun-Hsu Chou
Dr Jou Biotech Co., Ltd., No. 21, Lugong S. 2nd Road, Lukang Township, Changhua County 50544, Taiwan
Yu-Ying Wang
Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Road, South Dist., Taichung City 40227, Taiwan
Ping-Shan Lai
Department of Chemistry, National Chung Hsing University, No. 145, Xingda Road, Taichung 40227, Taiwan
Po-Cheng Chu
Department of Chemistry, National Chung Hsing University, No. 145, Xingda Road, Taichung 40227, Taiwan
Chang-Wei Hsieh
Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Road, South Dist., Taichung City 40227, Taiwan
Ultraviolet-A (UVA) exposure is a major cause of skin aging and can induce oxidative damage and accelerate skin wrinkling. Many natural polysaccharides exhibit a UV protective effect. In research on Pholiota nameko polysaccharides (PNPs), a natural macromolecular polysaccharide (4.4–333.487 kDa), studies have shown that PNPs can significantly decrease elastase activity to protect against UVA-induced aging in Hs68 human dermal fibroblasts. Cellular experiments in the present study indicated that PNPs can protect against UVA-induced oxidative damage in Hs68 cells by inhibiting the production of reactive oxygen species. Furthermore, PNPs significantly attenuated UVA-induced cell aging by decreasing the protein expression of matrix metalloproteinase 1, 3, and 9. Pretreatment of Hs68 cells with PNP-40, PNP-60, and PNP-80 before UVA irradiation increased protein expression of tissue inhibitor metalloproteinase 1 by 41%, 42%, and 56% relative to untreated cells. In conclusion, this study demonstrates that PNPs are a natural resource with potentially beneficial effects in protecting against UVA-induced skin aging.
