Application of a Fluorescent Probe for the Online Measurement of PM-Bound Reactive Oxygen Species in Chamber and Ambient Studies
Reece Brown,
Svetlana Stevanovic,
Zachary Brown,
Mingfu Cai,
Shengzhen Zhou,
Wei Song,
Xinming Wang,
Branka Miljevic,
Jun Zhao,
Steven Bottle,
Zoran Ristovski
Affiliations
Reece Brown
ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Svetlana Stevanovic
ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Zachary Brown
ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Mingfu Cai
School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China
Shengzhen Zhou
School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China
Wei Song
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry (GIG), Chinese Academy of Sciences, Guangzhou 510640, China
Xinming Wang
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry (GIG), Chinese Academy of Sciences, Guangzhou 510640, China
Branka Miljevic
ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Jun Zhao
School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China
Steven Bottle
School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology (QUT), Brisbane, 4000 QLD, Australia
Zoran Ristovski
ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
This manuscript details the application of a profluorescent nitroxide (PFN) for the online quantification of radical concentrations on particulate matter (PM) using an improved Particle Into Nitroxide Quencher (PINQ). A miniature flow-through fluorimeter developed specifically for use with the 9,10-bis(phenylethynyl)anthracene-nitroxide (BPEAnit) probe was integrated into the PINQ, along with automated gas phase corrections through periodic high efficiency particle arrestor (HEPA) filtering. The resulting instrument is capable of unattended sampling and was operated with a minimum time resolution of 2.5 min. Details of the fluorimeter design and examples of data processing are provided, and results from a chamber study of side-stream cigarette smoke and ambient monitoring campaign in Guangzhou, China are presented. Primary cigarette smoke was shown to have both short-lived (t1/2 = 27 min) and long-lived (t1/2 = indefinite) PM-bound reactive oxygen species (ROS) components which had previously only been observed in secondary organic aerosol (SOA).
