Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo

Naoki Tanimizu; Norihisa Ichinohe; Yasushi Sasaki; Tohru Itoh; Ryo Sudo; Tomoko Yamaguchi; Takeshi Katsuda; Takafumi Ninomiya; Takashi Tokino; Takahiro Ochiya; Atsushi Miyajima; Toshihiro Mitaka

doi:10.1038/s41467-021-23575-1

Nature Communications (Jun 2021)

Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo

Naoki Tanimizu,
Norihisa Ichinohe,
Yasushi Sasaki,
Tohru Itoh,
Ryo Sudo,
Tomoko Yamaguchi,
Takeshi Katsuda,
Takafumi Ninomiya,
Takashi Tokino,
Takahiro Ochiya,
Atsushi Miyajima,
Toshihiro Mitaka

Affiliations

Naoki Tanimizu: Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine
Norihisa Ichinohe: Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine
Yasushi Sasaki: Biology Division, Department of Liberal Arts and Sciences, Center for Medical Education, Sapporo Medical University
Tohru Itoh: Laboratory of Stem Cell Therapy, The Institute for Quantitative Biosciences, The University of Tokyo
Ryo Sudo: Department of System Design Engineering, Keio University
Tomoko Yamaguchi: Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University
Takeshi Katsuda: University of Pennsylvania Perelman School of Medicine
Takafumi Ninomiya: Department of Anatomy, Sapporo Medical University School of Medicine
Takashi Tokino: Department of Medical Genome Sciences, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine
Takahiro Ochiya: Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University
Atsushi Miyajima: Laboratory of Stem Cell Therapy, The Institute for Quantitative Biosciences, The University of Tokyo
Toshihiro Mitaka: Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine

DOI: https://doi.org/10.1038/s41467-021-23575-1
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Combining mouse hepatocyte progenitors and cholangiocytes ex vivo, the authors form an organoid that can drain bile ex vivo and transport metabolites, as in the liver.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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