Wellcome Open Research (May 2021)
SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay [version 2; peer review: 2 approved]
- Michael D. Buck,
- Enzo Z. Poirier,
- Ana Cardoso,
- Bruno Frederico,
- Johnathan Canton,
- Sam Barrell,
- Rupert Beale,
- Richard Byrne,
- Simon Caidan,
- Margaret Crawford,
- Laura Cubitt,
- Sonia Gandhi,
- Robert Goldstone,
- Paul R. Grant,
- Kiran Gulati,
- Steve Hindmarsh,
- Michael Howell,
- Michael Hubank,
- Rachael Instrell,
- Ming Jiang,
- George Kassiotis,
- Wei-Ting Lu,
- James I. MacRae,
- Iana Martini,
- Davin Miller,
- David Moore,
- Eleni Nastouli,
- Jerome Nicod,
- Luke Nightingale,
- Jessica Olsen,
- Amin Oomatia,
- Nicola O'Reilly,
- Anett Rideg,
- Ok-Ryul Song,
- Amy Strange,
- Charles Swanton,
- Samra Turajlic,
- Mary Wu,
- Caetano Reis e Sousa,
- The Crick COVID-19 Consortium
Affiliations
- Michael D. Buck
- The Francis Crick Institute, London, NW1 1AT, UK
- Enzo Z. Poirier
- The Francis Crick Institute, London, NW1 1AT, UK
- Ana Cardoso
- The Francis Crick Institute, London, NW1 1AT, UK
- Bruno Frederico
- The Francis Crick Institute, London, NW1 1AT, UK
- Johnathan Canton
- The Francis Crick Institute, London, NW1 1AT, UK
- Sam Barrell
- The Francis Crick Institute, London, NW1 1AT, UK
- Rupert Beale
- The Francis Crick Institute, London, NW1 1AT, UK
- Richard Byrne
- The Francis Crick Institute, London, NW1 1AT, UK
- Simon Caidan
- The Francis Crick Institute, London, NW1 1AT, UK
- Margaret Crawford
- The Francis Crick Institute, London, NW1 1AT, UK
- Laura Cubitt
- The Francis Crick Institute, London, NW1 1AT, UK
- Sonia Gandhi
- The Francis Crick Institute, London, NW1 1AT, UK
- Robert Goldstone
- The Francis Crick Institute, London, NW1 1AT, UK
- Paul R. Grant
- Healh Service Laboratories, London, WC1H 9AX, UK
- Kiran Gulati
- New England Biolabs, Ipswich, MA, USA
- Steve Hindmarsh
- The Francis Crick Institute, London, NW1 1AT, UK
- Michael Howell
- The Francis Crick Institute, London, NW1 1AT, UK
- Michael Hubank
- The Royal Marsden Hospital, Sutton, SM2 5NG, UK
- Rachael Instrell
- The Francis Crick Institute, London, NW1 1AT, UK
- Ming Jiang
- The Francis Crick Institute, London, NW1 1AT, UK
- George Kassiotis
- The Francis Crick Institute, London, NW1 1AT, UK
- Wei-Ting Lu
- The Francis Crick Institute, London, NW1 1AT, UK
- James I. MacRae
- The Francis Crick Institute, London, NW1 1AT, UK
- Iana Martini
- The Royal Free Hospital, London, NW3 2QG, UK
- Davin Miller
- New England Biolabs, Ipswich, MA, USA
- David Moore
- University College London, London, WC1E 6BT, UK
- Eleni Nastouli
- The Francis Crick Institute, London, NW1 1AT, UK
- Jerome Nicod
- The Francis Crick Institute, London, NW1 1AT, UK
- Luke Nightingale
- The Francis Crick Institute, London, NW1 1AT, UK
- Jessica Olsen
- The Francis Crick Institute, London, NW1 1AT, UK
- Amin Oomatia
- The Royal Free Hospital, London, NW3 2QG, UK
- Nicola O'Reilly
- The Francis Crick Institute, London, NW1 1AT, UK
- Anett Rideg
- The Francis Crick Institute, London, NW1 1AT, UK
- Ok-Ryul Song
- The Francis Crick Institute, London, NW1 1AT, UK
- Amy Strange
- The Francis Crick Institute, London, NW1 1AT, UK
- Charles Swanton
- University College London, London, WC1E 6BT, UK
- Samra Turajlic
- The Francis Crick Institute, London, NW1 1AT, UK
- Mary Wu
- The Francis Crick Institute, London, NW1 1AT, UK
- Caetano Reis e Sousa
- The Francis Crick Institute, London, NW1 1AT, UK
- The Crick COVID-19 Consortium
- DOI
- https://doi.org/10.12688/wellcomeopenres.16517.2
- Journal volume & issue
-
Vol. 6
Abstract
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP that is robust, reliable, repeatable, specific, and inexpensive.
