Biotecnología Vegetal (Dec 2019)
In vitro multiplication of ginger Zingiber officinale Roscoe, cv. ‘Grand Caiman’ in Temporary Immersion System
Abstract
Ginger (Zingiber officinale Roscoe) is a plant of great interest for its properties and uses. Although it has been in vitro propagated in semi-solid culture media, the use of Temporary Immersion Systems (TIS) could be an alternative to increase the number of plants. The research aimed to multiply Z. officinale in TIS. Four experiments were carried out for selecting the immersion time (5, 10 and 15 minutes), the immersion frequency (four immersions every three hours, two every 12 hours and one every 24 hours), the volume of medium culture by explant (30, 40 and 50 ml) and a comparison of the TIS system, with the conventional method of propagation in semi-solid culture medium. It was found that the immersion time did not influence the evaluated variables. With immersions every six hours (four per day) the highest multiplication coefficient, shoot length and number of roots were obtained. With 20 ml of culture medium per explant, the plants were observed chlorotic and no significant differences were found between 40 and 60 ml. The use of TIS with an immersion time of 5 minutes, an immersion frequency every 6 hours and a volume of culture medium of 40 ml / explant, improved the response obtained from ginger shoots grown in semi-solid culture medium. The use of SIT for in vitro multiplication of Zingiber officinale Roscoe cv. ‘Grand Cayman’ increases the multiplication coefficient and improves the characteristics of the plants obtained. Its use is recommended as an alternative to propagate this cultivar selected by producers from the canton of Guatuso, Alajuela, Costa Rica.
