GENETIC DIVERSITY IN SODIUM AZIDE INDUCED WHEAT MUTANTS STUDIED BY SSR MARKERS

Abstract

Mutations induced artificially way are one of the tools used to increase genetic variation in populations where genetic variation has been shrinking especially due to various reasons one of which is domestication. In this study, Simple Sequence Repeats (SSRs) markers were used to screen genetic diversity in sodium azide (NaN3) induced fourteen fourth-generation advanced wheat mutant lines. The mean values of polymorphism rate (29.44%), polymorphic information content (PIC; 0.82), marker index (MI; 1.95) and resolving power (Rp; 1.31) were calculated according to SSR marker profiles. Two SSRs, Xwmc170 and Xcfd6, were detected as the most polymorphic markers, Xgwm626 proved the highest PIC and MI values, and Xcfd6 gave the highest Rp value. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram classified 15 plants into four groups. The Principle Component Analysis (PCA) showed 88.9% of the total genetic variation. The results obtained in the present study might be useful for determining the efficiency of NaN3 for creating mutant wheat lines with enough genetic variability to implement wheat-breeding programs as germplasm resources.

Keywords

• bread wheat
• ssr markers
• pca
• sodium azide mutagenesis
• somatic embryogenesis
• upgma
• bread wheat
• sodium azide mutagenesis
• somatic embryogenesis
• ssr markers
• pca
• upgma