مجله بیوتکنولوژی کشاورزی (Nov 2023)
DNA fingerprinting in ecotypes of three marshmallow species using ISSR molecular marker
Abstract
Knowledge of genetic diversity is the basis of plant breeding and various aspects such as selection of cross parents, protection of germplasm and prevention of genetic erosion are important. The purpose of this research is to evaluate the genetic diversity and investigate the genetic relationships in the native ecotypes of marigold in Iran in order to be used in breeding projects and to protect the genetic reserves of this plant. Materials and methods: In this research, after extracting DNA from young leaves by CTAB method, the molecular diversity of 18 ecotypes of three species of marshmallow bud was investigated using 10 ISSR primers. Results: The total number of bands formed by the primers studied in this research varied between 11 and 23 for each primer. The number of polymorphic bands in this research for the primers was at least 7 and at most 21 bands. In this research, the PIC ranged between 0.19 and 0.42 and its average was 0.29. The highest PIC was related to the ISSR2 marker and the lowest PIC was related to the ISSR4 marker. Cluster analysis showed that at the similarity level of 65%, the ecotypes related to each species were in one group and the ecotypes related to different species were placed in separate groups, so that the ecotypes of Qazvin, Taft, Yazd, Sari, Kerman and Shiraz which All of them belonged to the Althaea Rosea species and were placed in one group. In the second group, the ecotypes belonging to the Althaea Ficifolia species, which included Behshahr, Gorgan, Bam, Rafsanjan, Hamadan and Mashhad, were placed.The results of molecular variance analysis showed that 28% of the variation is within the species and 72% of the variation is Among the studied species. The results of analysis to main coordinates also confirmed the results of cluster analysis. Conclusion; According to the obtained results, the ISSR marker and the primers investigated in this experiment have the necessary efficiency to distinguish the ecotypes and the species of khatami flowers, and on the other hand, due to the genetic diversity of the ecotypes studied in this experiment, it can be The title of the parents was used in the breeding projects of this plant, and in order to preserve the germplasm and prevent genetic erosion, it is suggested to keep the ecotypes studied in this research in plant gene bank .
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