HPLC–MS/MS method for the simultaneous determination of aflatoxins in blood: toxicokinetics of aflatoxin B1 and aflatoxin M1 in rats

Abstract

Abstract Mycotoxins are highly toxic fungal metabolites that can pose health threats to humans and animals. Aflatoxins are a type of mycotoxin produced mainly by Aspergillus flavus and A. parasiticus. A sensitive high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method with multiple reaction monitoring (MRM) modes was developed for the determination of aflatoxins in blood after acetonitrile precipitation extraction. The limits of quantification of aflatoxins ranged from 0.05 to 0.2 ng/mL. Intra-day accuracy ranged from 92 to 111.0%, and intra-day precision (n = 6) ranged from 1 to 8%. Inter-day accuracy and precision were 94.0–102.0% and 2.0–8.0%, respectively. The toxicokinetics of AFB1 and its metabolite AFM1 after a single oral administration (AFB1 1 mg/kg body weight) were studied in male Sprague–Dawley rats. The blood AFB1 and AFM1 profiles could be adequately described by a noncompartmental model. The highest concentration of AFB1 (C max 93.42 ± 23.01 ng/mL) was observed with T max at 0.15 ± 0.034 h. AFB1 was rapidly metabolized to AFM1 which reached its peak blood concentration (C max 53.86 ± 12.12 ng/mL) at 0.33 ± 0.11 h. The HPLC–MS/MS method was simple and sensitive, appropriate for studying the in vivo toxicokinetics of aflatoxins.

Keywords

• Aflatoxins
• AFB1
• AFM1
• HPLC–MS/MS
• Toxicokinetic